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Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine islet‐like clusters

Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine... Background Intraportal infusion is currently the method of choice for clinical islet cell transplantation but suffers from poor efficacy. As the liver may not represent an optimal transplantation site for Langerhans islets, we examined the potential of neonatal porcine islet‐like clusters (NPICCs) to engraft in skeletal muscle as an alternative transplantation site. Methods Neonatal porcine islet‐like clusters were isolated from 2‐ to 5‐day‐old piglets and either transplanted under the kidney capsule (s.k.) or injected into the lower hindlimb muscle (i.m.) of streptozotocin‐diabetic NOD‐SCID IL2rγ−/− (NSG) mice. Survival, vascularization, maturation, and functional activity were analyzed by intraperitoneal glucose tolerance testing and immunohistochemical analyses. Results Intramuscular transplantation of NPICCs resulted in development of normoglycemia and restored glucose homeostasis. Time to reversal of diabetes and glucose tolerance (AUC glucose and AUC insulin) did not significantly differ as compared to s.k. transplantation. Intramuscular grafts exhibited rapid neovascularization and graft composition with cytokeratin‐positive ductal cells and beta cells at post‐transplant weeks 2 and 8 and after establishment of normoglycemia was comparable in both groups. Conclusions Intramuscular injection represents a minimally invasive but efficient alternative for transplantation of NPICCs and, thus, offers an attractive alternative site for xenotransplantation approaches. These findings may have important implications for improving the outcome and the monitoring of pig islet xenotransplantation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Engraftment and reversal of diabetes after intramuscular transplantation of neonatal porcine islet‐like clusters

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References (30)

Publisher
Wiley
Copyright
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12201
pmid
26490671
Publisher site
See Article on Publisher Site

Abstract

Background Intraportal infusion is currently the method of choice for clinical islet cell transplantation but suffers from poor efficacy. As the liver may not represent an optimal transplantation site for Langerhans islets, we examined the potential of neonatal porcine islet‐like clusters (NPICCs) to engraft in skeletal muscle as an alternative transplantation site. Methods Neonatal porcine islet‐like clusters were isolated from 2‐ to 5‐day‐old piglets and either transplanted under the kidney capsule (s.k.) or injected into the lower hindlimb muscle (i.m.) of streptozotocin‐diabetic NOD‐SCID IL2rγ−/− (NSG) mice. Survival, vascularization, maturation, and functional activity were analyzed by intraperitoneal glucose tolerance testing and immunohistochemical analyses. Results Intramuscular transplantation of NPICCs resulted in development of normoglycemia and restored glucose homeostasis. Time to reversal of diabetes and glucose tolerance (AUC glucose and AUC insulin) did not significantly differ as compared to s.k. transplantation. Intramuscular grafts exhibited rapid neovascularization and graft composition with cytokeratin‐positive ductal cells and beta cells at post‐transplant weeks 2 and 8 and after establishment of normoglycemia was comparable in both groups. Conclusions Intramuscular injection represents a minimally invasive but efficient alternative for transplantation of NPICCs and, thus, offers an attractive alternative site for xenotransplantation approaches. These findings may have important implications for improving the outcome and the monitoring of pig islet xenotransplantation.

Journal

XenotransplantationWiley

Published: Nov 1, 2015

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