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Effect of leucine‐to‐methionine substitutions on the diffraction quality of histone chaperone SET/TAF‐Iβ/INHAT crystals

Effect of leucine‐to‐methionine substitutions on the diffraction quality of histone chaperone... One of the most frequent problems in crystallization is poor quality of the crystals. In order to overcome this obstacle several methods have been utilized, including amino‐acid substitutions of the target protein. Here, an example is presented of crystal‐quality improvement by leucine‐to‐methionine substitutions. A variant protein with three amino‐acid substitutions enabled improvement of the crystal quality of the histone chaperone SET/TAF‐Iβ/INHAT when combined with optimization of the cryoconditions. This procedure improved the resolution of the SET/TAF‐Iβ/INHAT crystals from around 5.5 to 2.3 Å without changing the crystallization conditions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Effect of leucine‐to‐methionine substitutions on the diffraction quality of histone chaperone SET/TAF‐Iβ/INHAT crystals

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References (8)

Publisher
Wiley
Copyright
International Union of Crystallography, 2008
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309108028704
pmid
18931446
Publisher site
See Article on Publisher Site

Abstract

One of the most frequent problems in crystallization is poor quality of the crystals. In order to overcome this obstacle several methods have been utilized, including amino‐acid substitutions of the target protein. Here, an example is presented of crystal‐quality improvement by leucine‐to‐methionine substitutions. A variant protein with three amino‐acid substitutions enabled improvement of the crystal quality of the histone chaperone SET/TAF‐Iβ/INHAT when combined with optimization of the cryoconditions. This procedure improved the resolution of the SET/TAF‐Iβ/INHAT crystals from around 5.5 to 2.3 Å without changing the crystallization conditions.

Journal

Acta Crystallographica Section FWiley

Published: Oct 1, 2008

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