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N. Kirchhof, S. Shibata, M. Wijkstrom, D. Kulick, C. Salerno, S. Clemmings, Y. Heremans, U. Galili, D. Sutherland, A. Dalmasso, B. Hering (2004)
Reversal of diabetes in non‐immunosuppressed rhesus macaques by intraportal porcine islet xenografts precedes acute cellular rejectionXenotransplantation, 11
J. Pratt, Shamim Basheer, S. Sacks (2002)
Local synthesis of complement component C3 regulates acute renal transplant rejectionNature Medicine, 8
A. Soulika, M. Khan, T. Hattori, F. Bowen, B. Richardson, C. Hack, A. Sahu, L. Edmunds, John Lambris (2000)
Inhibition of heparin/protamine complex-induced complement activation by Compstatin in baboons.Clinical immunology, 96 3
H. Brandhorst, D. Brandhorst, B. Hering, R. Bretzel (1999)
Significant progress in porcine islet mass isolation utilizing liberase HI for enzymatic low-temperature pancreas digestion.Transplantation, 68 3
H. Johansson, M. Goto, A. Siegbahn, G. Elgue, O. Korsgren, B. Nilsson (2006)
Low Molecular Weight Dextran Sulfate: A Strong Candidate Drug to Block IBMIR in Clinical Islet TransplantationAmerican Journal of Transplantation, 6
M. Katragadda, P. Magotti, G. Sfyroera, John Lambris (2006)
Hydrophobic effect and hydrogen bonds account for the improved activity of a complement inhibitor, compstatin.Journal of medicinal chemistry, 49 15
L. Fernandez, E. Hatch, B. Armann, J. Odorico, D. Hullett, H. Sollinger, M. Hanson (2005)
Validation of Large Particle Flow Cytometry for the Analysis and Sorting of Intact Pancreatic IsletsTransplantation, 80
K. Brown, E. Kondeatis, R. Vaughan, S. Kon, C. Farmer, John Taylor, Xiang He, A. Johnston, C. Horsfield, B. Janssen, P. Gros, Wuding Zhou, S. Sacks, N. Sheerin (2006)
Influence of donor C3 allotype on late renal-transplantation outcome.The New England journal of medicine, 354 19
Bo Nilsson, Rolf Larsson, Jaan Hong, G. Elgue, K. Ekdahl, A. Sahu, John Lambris (1998)
Compstatin inhibits complement and cellular activation in whole blood in two models of extracorporeal circulation.Blood, 92 5
P. Rood, L. Buhler, R. Bottino, M. Trucco, D. Cooper (2006)
Pig-to-nonhuman primate islet xenotransplantation: a review of current problems.Cell transplantation, 15 2
K. Cardona, G. Korbutt, Z. Milas, J. Lyon, J. Cano, Wan-lin Jiang, Hameeda Bello-Laborn, Brad Hacquoil, E. Strobert, Shivaprakash Gangappa, C. Weber, T. Pearson, R. Rajotte, C. Larsen (2006)
Long-term survival of neonatal porcine islets in nonhuman primates by targeting costimulation pathwaysNature Medicine, 12
M. Goto, C. Groth, B. Nilsson, O. Korsgren (2004)
Intraportal pig islet xenotransplantation into athymic mice as an in vivo model for the study of the instant blood‐mediated inflammatory reactionXenotransplantation, 11
B. Hering, M. Wijkstrom, M. Graham, M. Hårdstedt, T. Aasheim, T. Jie, J. Ansite, M. Nakano, Jane Cheng, Wei Li, K. Moran, U. Christians, Colleen Finnegan, C. Mills, D. Sutherland, P. Bansal‐Pakala, M. Murtaugh, N. Kirchhof, H. Schuurman (2006)
Prolonged diabetes reversal after intraportal xenotransplantation of wild-type porcine islets in immunosuppressed nonhuman primatesNature Medicine, 12
K. Ekdahl, B. Nilsson, M. Pekna, U. Nilsson (1992)
Generation of iC3 at the Interface between Blood and GasScandinavian Journal of Immunology, 35
W. Bennet, B. Sundberg, T. Lundgren, A. Tibell, C. Groth, A. Richards, D. White, G. Elgue, R. Larsson, B. Nilsson, O. Korsgren (2000)
Damage to porcine islets of Langerhans after exposure to human blood in vitro, or after intraportal transplantation to cynomologus monkeys: protective effects of sCR1 and heparin.Transplantation, 69 5
O. Hamad, K. Ekdahl, Per Nilsson, J. Andersson, P. Magotti, John Lambris, Bo Nilsson (2008)
Complement activation triggered by chondroitin sulfate released by thrombin receptor‐activated plateletsJournal of Thrombosis and Haemostasis, 6
J. Kissane, E. Robins (1958)
The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system.The Journal of biological chemistry, 233 1
J. A.M., Ames, Hapiro, Onathan, L. R.T, Akey, Dmond, R. A., Yan, regory, K. S, Orbutt, Arth, W. L., Arnock, Orman, K. M., Neteman, Ay, R. V, Ajotte (2000)
Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive Regimen
Patrizia Fiorante, Y. Banz, P. Mohacsi, A. Kappeler, W. Wuillemin, P. Macchiarini, A. Roos, M. Daha, T. Schaffner, A. Haeberli, G. Mazmanian, R. Rieben (2001)
Low molecular weight dextran sulfate prevents complement activation and delays hyperacute rejection in pig‐to‐human xenotransplantation modelsXenotransplantation, 8
H. Thomas, F. Maillet, D. Letourneur, J. Jozefonvicz, E. Fischer, M. Kazatchkine (1996)
Sulfonated dextran inhibits complement activation and complement-dependent cytotoxicity in an in vitro model of hyperacute xenograft rejection.Molecular immunology, 33 7-8
T. Mollnes, J. Riesenfeld, P. Garred, E. Nordström, K. Høgåsen, E. Fosse, O. Götze, M. Harboe (1995)
A new model for evaluation of biocompatibility: combined determination of neoepitopes in blood and on artificial surfaces demonstrates reduced complement activation by immobilization of heparin.Artificial organs, 19 9
P. Chowdhury, Wuding Zhou, S. Sacks (2003)
Complement in Renal TransplantationNephron Clinical Practice, 95
H. Thomas, F. Maillet, D. Letourneur, J. Jozefonvicz, M. Kazatchkine, E. Fischer (1996)
A synthetic dextran derivative inhibits complement activation and complement-mediated cytotoxicity in an in vitro model of hyperacute xenograft rejection.Transplantation proceedings, 28 2
(2000)
to cynomologus monkeys : protective effects of sCR 1 and heparin
M. Goto, H. Johansson, A. Maeda, G. Elgue, O. Korsgren, B. Nilsson (2004)
Low molecular weight dextran sulfate prevents the instant blood-mediated inflammatory reaction induced by adult porcine isletsTransplantation, 77
P. Rood, R. Bottino, A. Balamurugan, C. Smetanka, D. Ayares, C. Groth, N. Murase, D. Cooper, M. Trucco (2007)
Reduction of Early Graft Loss After Intraportal Porcine Islet Transplantation in MonkeysTransplantation, 83
D. Cantarovich, G. Blancho, N. Potiron, N. Jugeau, M. Fiche, C. Chagneau, E. Letessier, F. Boeffard, P. Loth, G. Karam, J. Soulillou, B. Mauff (2002)
Rapid failure of pig islet transplantation in non human primatesXenotransplantation, 9
L. Bühler, S. Deng, J. O'neil, H. Kitamura, M. Koulmanda, A. Baldi, J. Rahier, I. Alwayn, J. Appel, M. Awwad, D. Sachs, G. Weir, J. Squifflet, D. Cooper, P. Morel (2002)
Adult porcine islet transplantation in baboons treated with conventional immunosuppression or a non‐myeloablative regimen and CD154 blockadeXenotransplantation, 9
Q. Peng, Ke Li, H. Patel, S. Sacks, Wuding Zhou (2006)
Dendritic Cell Synthesis of C3 Is Required for Full T Cell Activation and Development of a Th1 Phenotype1The Journal of Immunology, 176
Lisa Ozmen, K. Ekdahl, G. Elgue, R. Larsson, O. Korsgren, B. Nilsson (2002)
Inhibition of thrombin abrogates the instant blood-mediated inflammatory reaction triggered by isolated human islets: possible application of the thrombin inhibitor melagatran in clinical islet transplantation.Diabetes, 51 6
Abstract: Background: A massive destruction of transplanted tissue occurs immediately following transplantation of pancreatic islets from pig to non‐human primates. The detrimental instant blood‐mediated inflammatory reaction (IBMIR), triggered by the porcine islets, is a likely explanation for this tissue loss. This reaction may also be responsible for mediating an adaptive immune response in the recipient that requires a heavy immunosuppressive regimen. Materials and methods: Low molecular weight dextran sulfate (LMW‐DS) and the complement inhibitor Compstatin were used in a combination of in vitro and in vivo studies designed to dissect the xenogeneic IBMIR in a non‐human primate model of pancreatic islet transplantation. Adult porcine islets (10 000 IEQs/kg) were transplanted intraportally into three pairs of cynomolgus monkeys that had been treated with LMW‐DS or heparin (control), and the effects on the IBMIR were characterized. Porcine islets were also incubated in human blood plasma in vitro to assess complement inhibition by LMW‐DS and Compstatin. Results: Morphological scoring and immunohistochemical staining revealed that the severe islet destruction and macrophage, neutrophilic granulocyte, and T‐cell infiltration observed in the control (heparin‐treated) animals were abrogated in the LMW‐DS‐treated monkeys. Both coagulation and complement activation were significantly reduced in monkeys treated with LMW‐DS, but IgM and complement fragments were still found on the islet surface. This residual complement activation could be inhibited by Compstatin in vitro. Conclusions: The xenogeneic IBMIR in this non‐human primate model is characterized by an immediate binding of antibodies that triggers deleterious complement activation and a subsequent clotting reaction that leads to further complement activation. The effectiveness of LMW‐DS (in vivo and in vitro) and Compstatin (in vitro) in inhibiting this IBMIR provides the basis for a protocol that can be used to abrogate the IBMIR in pig‐human clinical islet transplantation.
Xenotransplantation – Wiley
Published: Jul 1, 2008
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