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Direct methods in protein electron crystallography – beef liver catalase in its fully hydrated form at room temperature

Direct methods in protein electron crystallography – beef liver catalase in its fully hydrated... The crystal structure of beef liver catalase was determined abinitio in projection to 9 Å resolution using electron diffraction data at room temperature from hydrated specimens maintained in an environmental chamber in the electron microscope. A conserva­tive combination of symbolic addition with maximum entropy and likelihood led to a model with a Patterson correlation coefficient to the observed data. This independent solution could then be compared favorably to a previous 23 Å analysis of electron micrographs from frozen hydrated preparations. Prediction of the higher‐resolution structure by extension of the lower‐resolution image‐based phase basis set also gave a good match to the direct‐methods solution, particularly for the most intense reflections. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section A Foundations of Crystallography Wiley

Direct methods in protein electron crystallography – beef liver catalase in its fully hydrated form at room temperature

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References (40)

Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0108-7673
eISSN
1600-5724
DOI
10.1107/S0108767398010836
Publisher site
See Article on Publisher Site

Abstract

The crystal structure of beef liver catalase was determined abinitio in projection to 9 Å resolution using electron diffraction data at room temperature from hydrated specimens maintained in an environmental chamber in the electron microscope. A conserva­tive combination of symbolic addition with maximum entropy and likelihood led to a model with a Patterson correlation coefficient to the observed data. This independent solution could then be compared favorably to a previous 23 Å analysis of electron micrographs from frozen hydrated preparations. Prediction of the higher‐resolution structure by extension of the lower‐resolution image‐based phase basis set also gave a good match to the direct‐methods solution, particularly for the most intense reflections.

Journal

Acta Crystallographica Section A Foundations of CrystallographyWiley

Published: Jan 1, 1999

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