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Development and characterization of 13 microsatellite markers of Chanodichthys mongolicus (Cypriniformes: Cyprinidae) by RAD‐seq

Development and characterization of 13 microsatellite markers of Chanodichthys mongolicus... This study developed and characterized 13 novel polymorphic microsatellite markers from Mongolian redfin Chanodichthys mongolicus by restriction‐site associated DNA sequencing (RAD‐seq). Variability was tested on 36 individuals randomly collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 (MGB_1990, MGB_3456, MGB_6007, MGB_6539, MGB_7273, MGB_8632) to 9 (MGB_8632). Observed heterozygosity ranged from 0.000 (MGB_7273) to 0.972 (MGB_5057), whereas the expected heterozygosity ranged from 0.028 (MGB_6007) to 0.795 (MGB_4875). Polymorphism Information Content ranged from 0.027 (MGB_6007) to 0.755 (MGB_4875). Moreover, seven microsatellites were in agreement with Hardy‐Weinberg equilibrium. Four pairwise tests in seven microsatellite loci (MGB_1498, MGB_1990, MGB_4875, MGB_5057, MGB_6539, MGB_7273, MGB_8632) indicated linkage disequilibrium among loci. These microsatellites can evaluate Mongolian redfin populations’ genetic parameters to provide precious information for a conservation management system. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Ichthyology Wiley

Development and characterization of 13 microsatellite markers of Chanodichthys mongolicus (Cypriniformes: Cyprinidae) by RAD‐seq

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References (27)

Publisher
Wiley
Copyright
Copyright © 2021 Blackwell Verlag GmbH
ISSN
0175-8659
eISSN
1439-0426
DOI
10.1111/jai.14253
Publisher site
See Article on Publisher Site

Abstract

This study developed and characterized 13 novel polymorphic microsatellite markers from Mongolian redfin Chanodichthys mongolicus by restriction‐site associated DNA sequencing (RAD‐seq). Variability was tested on 36 individuals randomly collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 (MGB_1990, MGB_3456, MGB_6007, MGB_6539, MGB_7273, MGB_8632) to 9 (MGB_8632). Observed heterozygosity ranged from 0.000 (MGB_7273) to 0.972 (MGB_5057), whereas the expected heterozygosity ranged from 0.028 (MGB_6007) to 0.795 (MGB_4875). Polymorphism Information Content ranged from 0.027 (MGB_6007) to 0.755 (MGB_4875). Moreover, seven microsatellites were in agreement with Hardy‐Weinberg equilibrium. Four pairwise tests in seven microsatellite loci (MGB_1498, MGB_1990, MGB_4875, MGB_5057, MGB_6539, MGB_7273, MGB_8632) indicated linkage disequilibrium among loci. These microsatellites can evaluate Mongolian redfin populations’ genetic parameters to provide precious information for a conservation management system.

Journal

Journal of Applied IchthyologyWiley

Published: Dec 1, 2021

Keywords: Chanodichthys mongolicus; microsatellites; polymorphism; restriction‐site associated DNA sequencing

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