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Goodwin Ah, D. Cooper, A. Malcolm, Ippolito Rm, E. Koren, F. Neethling, Y. Ye, N. Zuhdi, Lamontagne Lr (1992)
Identification of carbohydrate structures that bind human antiporcine antibodies: implications for discordant xenografting in humans.Transplantation proceedings, 24 2
U. Galili, F. Anaraki, A. Thall, C. Hill-Black, M. Radic (1993)
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D. Cooper, Goodwin Ah, E. Koren, R. Oriol, Malcolm Aj, Ippolito Rm, F. Neethling, Y. Ye, E. Romano, N. Zuhdi (1993)
Identification of alpha-galactosyl and other carbohydrate epitopes that are bound by human anti-pig antibodies: relevance to discordant xenografting in man.Transplant immunology, 1 3
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D. Cooper, E. Koren, R. Oriol (1994)
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D. Cooper, Y. Ye, M. Niekrasz, M. Kehoe, M. Martin, F. Neethling, S. Kosanke, L. Debault, G. Worsley, N. Zuhdi, R. Oriol, E. Romano (1993)
SPECIFIC INTRAVENOUS CARBOHYDRATE THERAPY A NEW CONCEPT IN INHIBITING ANTIBODY‐MEDIATED REJECTION—EXPERIENCE WITH ABO‐INCOMPATIBLE CARDIAC ALLOGRAFTING IN THE BABOONTransplantation, 56
N. Bovin (1993)
Sugar-Polyacrylamide Conjugates as Probes for Cell Lectins
E. Koren, F. Neethling, M. Koscec, M. Kujundžić, S. Richards, Y. Ye, R. Oriol, D. Cooper (1994)
In vitro model for hyperacute rejection of xenogeneic cells.Transplantation proceedings, 26 3
M. Sandrin, H. Vaughan, P. Dabkowski, I. McKenzie (1993)
Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes.Proceedings of the National Academy of Sciences of the United States of America, 90 23
Bannett Ad, McAlack Rf, Rasib Raja, A. Baquero, M. Morris (1987)
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E. Koren, F. Neethling, S. Richards, M. Koscec, Y. Ye, N. Zuhdi, D. Cooper (1993)
Binding and specificity of major immunoglobulin classes of preformed human anti-pig heart antibodiesTransplant International, 6
Abstract: The presence of naturally occurring anti‐Galα1–3Gal (anti‐αGal) antibodies in human serum is believed to be a major factor in the hyperacute rejection of discordant organ xenografts such as the pig‐to‐human combination. Galα1–3Gal epitopes are expressed on pig tissues and the binding of anti‐αGal leads to endothelial cell activation and complement‐mediated, hyperacute graft rejection. One possible method to overcome this problem is to absorb anti‐αGal antibodies from the plasma of the xenograft recipient using a suitable immunoabsorbent or to interfere with their binding to tissues and thus prevent their cytotoxic activity by the intravenous injection of soluble antigen. We describe here the use of new synthetic antigens containing the Galα1–3Gal disaccharide (Bdi) epitope. Soluble conjugates of the Bdi with polyacrylamide (PAA‐Bdi) were used as coating antigens for an anti‐αGal ELISA as well as for in vitro inhibition of the cytotoxicity of anti‐αGal. An immunoabsorbent consisting of PAA‐Bdi coupled to macroporous glass (Sorbent Bdi) was tested for absorption of anti‐αGal from human serum. Anti‐αGal IgM, IgG and IgA could be detected by the anti‐αGal ELISA and were specifically absorbed by Sorbent Bdi with absorption efficiencies ranging from 70 to 50% for anti‐αGal IgG and 60 to 25% for anti‐αGal IgM. A comparison of the anti‐αGal absorption by Sorbent Bdi and rabbit red blood cells revealed a qualitatively (isotype distribution) and quantitatively similar pattern. Nonspecific absorption by Sorbent Bdi was low, as detected by the reduction of anti‐A trisaccharide antibodies. The cytotoxic effect of human serum on pig kidney (PK15) cells was almost totally inhibited by the addition of synthetic B disaccharide or by adsorption of the serum through immunoaffinity columns of PAA‐Bdi. We conclude that the newly developed, synthetic αGal1–3Gal antigens are suitable for the detection and immunoabsorption or inhibition of anti‐αGal antibodies.
Xenotransplantation – Wiley
Published: May 1, 1995
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