Access the full text.
Sign up today, get DeepDyve free for 14 days.
F. Neethling, E. Koren, Y. Ye, Steven Richards, M. Kujundžić, R. Oriol, D. Cooper (1994)
PROTECTION OF PIG KIDNEY (PK15) CELLS FROM THE CYTOTOXIC EFFECT OF ANTI‐PIG ANTIBODIES BY α‐GALACTOSYL OLIGOSACCHARIDES1Transplantation, 57
U. Galili, Rachmilewitz Ea, A. Peleg, I. Flechner (1984)
A unique natural human IgG antibody with anti-alpha-galactosyl specificityThe Journal of Experimental Medicine, 160
M. Romanella, A. Aminian, W. Adam, M. Pearse, A. D'apice (1997)
Involvement of both the classical and alternate pathways of complement in an ex vivo model of xenograft rejection.Transplantation, 63 7
I. Grupp, A. Subramaniam, T. Hewett, J. Robbins, G. Grupp (1993)
Comparison of normal, hypodynamic, and hyperdynamic mouse hearts using isolated work-performing heart preparations.The American journal of physiology, 265 4 Pt 2
Sandrin Sandrin, Vaughan Vaughan, Dabkowski Dabkowski, McKenzie McKenzie (1993)
Anti‐pig IgM antibodies in human serum react predominantly with Gal(α‐3)Gal epitopesProc Natl Acad Sci US A, 90
R. Tearle, M. Tange, Z. Zannettino, Marina Katerelos, T. Shinkel, B. Denderen, A. Lonie, I. Lyons, M. Nottle, T. Cox, C. Becker, A. Peura, P. Wigley, Robert Crawford, Allan Robins, Martin Pearse, A. D'apice (1996)
The alpha-1,3-galactosyltransferase knockout mouse. Implications for xenotransplantation.Transplantation, 61 1
R. Oriol, Y. Ye, E. Koren, D. Cooper (1993)
CARBOHYDRATE ANTIGENS OF PIG TISSUES REACTING WITH HUMAN NATURAL ANTIBODIES AS POTENTIAL TARGETS FOR HYPERACUTE VASCULAR REJECTION IN PIG‐TO‐MAN ORGAN XENOTRANSPLANTATION1Transplantation, 56
D. Cooper, Goodwin Ah, E. Koren, R. Oriol, Malcolm Aj, Ippolito Rm, F. Neethling, Y. Ye, E. Romano, N. Zuhdi (1993)
Identification of alpha-galactosyl and other carbohydrate epitopes that are bound by human anti-pig antibodies: relevance to discordant xenografting in man.Transplant immunology, 1 3
Cooper (1996)
Extracorporeal immunoabsorption of aGal antibodiesXenotransplantation, 4
B. Collins, W. Parker, J.L PIatt (1994)
Characterization of porcine endothelial cell determinants recognized by human natural antibodiesXenotransplantation, 1
R Larsen, C Rivera-Marrero, L. Ernst, R Cummings, J. Lowe (1990)
Frameshift and nonsense mutations in a human genomic sequence homologous to a murine UDP-Gal:beta-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase cDNA.The Journal of biological chemistry, 265 12
Y. Ye, F. Neethling, M. Niekrasz, Eugen Koren, S. Richards, M. Martin, Stanley Kosanke, Rafael Oriol, David Cooper (1994)
Evidence that intravenously administered alpha-galactosyl carbohydrates reduce baboon serum cytotoxicity to pig kidney cells (PK15) and transplanted pig hearts.Transplantation, 58 3
Hull Hull, Cherry Cherry, Weaver Weaver (1976)
The origin and characteristics of a pig kidney cell strain LLC‐PK1Vitro, 12
M. Sandrin, H. Vaughan, P. Dabkowski, I. McKenzie (1993)
Anti-pig IgM antibodies in human serum react predominantly with Gal(alpha 1-3)Gal epitopes.Proceedings of the National Academy of Sciences of the United States of America, 90 23
U. Galili, R. Mandrell, Randa Hamadeh, S. Shohet, J. Griffiss (1988)
Interaction between human natural anti-alpha-galactosyl immunoglobulin G and bacteria of the human floraInfection and Immunity, 56
Abstract: The galactose a 1‐3 galactose terminal disaccharide (Gal epitope) has been identified as the major porcine xenoantigen recognised by xenoantibody in human plasma. Elimination or suppression of the epitope or antibody will be a major factor in overcoming hyperacute rejection. Inhibition of the antibody by depletion or elimination of the epitope by gene knockout may reveal the importance of other xenoantibodies, and in addition elimination of the epitope may unmask or produce other xenoantibody combinations. This study aims to determine the relative importance of anti‐Gal antibody and Gal epitope elimination in a functional model of xenotransplantation, ex vivo perfusion of mouse hearts with human plasma on a Langendorff apparatus. Perfusion of mouse hearts with human plasma depleted of anti‐Gal antibody demonstrates a protective effect compared to hearts perfused with undepleted plasma with prolongation of survival time from 24.1 to 44.5 min. Similarly, elimination of the epitope is also protective. Hearts from Gal knockout mice, which were generated by gene targeting of the al,3 galactosyltransferase gene, and hearts from appropriate control mice were perfused with human plasma. Gal knockout mice hearts demonstrated an increase in survival time from 10.2 to 33.8 min compared to control hearts. This was accompanied by a decrease in C3c and IgM, but little change in IgG deposition. The protective effect is incomplete, probably due to the effect of antibodies against non‐Gal xenoantigens. There was no functional evidence for generation of neo‐antigens in the Gal KO mice that were I recognised by naturally occurring human xenoantibodies.
Xenotransplantation – Wiley
Published: Feb 1, 1997
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.