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- Publisher
- Wiley
- Copyright
- International Union of Crystallography, 2011
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309111025929
- pmid
- 22102028
- Publisher site
- See Article on Publisher Site
Abstract
Actinomyces oris is an oral bacterium important for the development of dental plaque. It expresses two forms of fimbriae: type 1 and type 2. FimP, which is the fimbrial protein that is polymerized into the stalk of the type 1 fimbriae, was cloned, overexpressed and crystallized. X‐ray data were collected and processed to 2.2 Å resolution. The crystals belonged to space group P21212, with one molecule in the asymmetric unit. To facilitate structure determination using single anomalous dispersion, three methionines were introduced by site‐directed mutagenesis. Crystals of selenomethionine‐labelled protein were obtained by streak‐seeding and diffracted to 2.0 Å resolution.
Journal
Acta Crystallographica Section F – Wiley
Published: Oct 1, 2011