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Crystallization and X‐ray analysis of the salmon‐egg lectin SEL24K

Crystallization and X‐ray analysis of the salmon‐egg lectin SEL24K The 24 kDa egg lectin of Chinook salmon (Oncorhynchus tshawytscha) is released from the egg during the cortical reaction. The lectin functions in blocking polyspermy during the fertilization process. The egg lectin was purified by affinity chromatography from salmon eggs and crystallized by the hanging‐drop vapor‐diffusion method using 15/4 EO/OH (pentaerythritol ethoxylate) as a precipitant. The crystal diffracted synchrotron‐radiation X‐rays to 1.63 Å resolution. The crystal belongs to the monoclinic space group P21, with unit‐cell parameters a = 93.0, b = 73.6, c = 113.6 Å, α = 90, β = 92.82, γ = 90°. The crystal is likely to contain eight molecules in the asymmetric unit (VM = 2.3 Å3 Da−1), corresponding to a solvent content of 45.5%. A self‐rotation function suggests an arrangement with 222 point symmetry within the asymmetric unit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and X‐ray analysis of the salmon‐egg lectin SEL24K

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References (9)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309107015345
pmid
17565179
Publisher site
See Article on Publisher Site

Abstract

The 24 kDa egg lectin of Chinook salmon (Oncorhynchus tshawytscha) is released from the egg during the cortical reaction. The lectin functions in blocking polyspermy during the fertilization process. The egg lectin was purified by affinity chromatography from salmon eggs and crystallized by the hanging‐drop vapor‐diffusion method using 15/4 EO/OH (pentaerythritol ethoxylate) as a precipitant. The crystal diffracted synchrotron‐radiation X‐rays to 1.63 Å resolution. The crystal belongs to the monoclinic space group P21, with unit‐cell parameters a = 93.0, b = 73.6, c = 113.6 Å, α = 90, β = 92.82, γ = 90°. The crystal is likely to contain eight molecules in the asymmetric unit (VM = 2.3 Å3 Da−1), corresponding to a solvent content of 45.5%. A self‐rotation function suggests an arrangement with 222 point symmetry within the asymmetric unit.

Journal

Acta Crystallographica Section F – Wiley

Published: May 1, 2007

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