Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Kataoka, Masahisa Ikemi, Tadashi Morikawa, T. Miyoshi, Ken-ichi Nishi, Masura Wada, Hideaki Yamada, Hideaki Yamada, Sakayu Shimizu (1997)
Isolation and characterization of D-threonine aldolase, a pyridoxal-5'-phosphate-dependent enzyme from Arthrobacter sp. DK-38.European journal of biochemistry, 248 2
M. Uhl, G. Oberdorfer, G. Steinkellner, L. Riegler-Berket, D. Mink, Friso Assema, Martin Schürmann, K. Gruber (2015)
The Crystal Structure of D-Threonine Aldolase from Alcaligenes xylosoxidans Provides Insight into a Metal Ion Assisted PLP-Dependent MechanismPLoS ONE, 10
Kateryna Fesko (2016)
Threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificitiesApplied Microbiology and Biotechnology, 100
(2017)
D-Threonine aldolase 89 Figure 3 X-ray diffraction image of a CrDTA crystal
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
P. Evans
Biological Crystallography Scaling and Assessment of Data Quality
Ji-Quan Liu, M. Odani, T. Yasuoka, T. Dairi, N. Itoh, M. Kataoka, Sakayu Shimizu, Hideaki Yamada (2000)
Gene cloning and overproduction of low-specificity d-threonine aldolase from Alcaligenes xylosoxidans and its application for production of a key intermediate for parkinsonism drugApplied Microbiology and Biotechnology, 54
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
A. Vagin, A. Teplyakov (2010)
Molecular replacement with MOLREP.Acta crystallographica. Section D, Biological crystallography, 66 Pt 1
Y. Hirato, Mayumi Tokuhisa, M. Tanigawa, H. Ashida, Hiroyuki Tanaka, K. Nishimura (2017)
Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii.Phytochemistry, 135
M. Winn, C. Ballard, K. Cowtan, E. Dodson, P. Emsley, P. Evans, R. Keegan, E. Krissinel, A. Leslie, A. Mccoy, S. McNicholas, G. Murshudov, N. Pannu, E. Potterton, H. Powell, R. Read, A. Vagin, K. Wilson (2011)
Overview of the CCP4 suite and current developmentsActa Crystallographica Section D: Biological Crystallography, 67
Ji‐Quan Liu, T. Dairi, N. Itoh, M. Kataoka, S. Shimizu, H. Yamada (2000)
Diversity of microbial threonine aldolases and their applicationJournal of Molecular Catalysis B-enzymatic, 10
S. Goldberg, A. Goswami, Zhiwei Guo, Y. Chan, Ehrlic Lo, Andrew Lee, V. Truc, K. Natalie, C. Hang, L. Rossano, Michael Schmidt (2015)
Preparation of β-hydroxy-α-amino Acid Using Recombinant d-Threonine AldolaseOrganic Process Research & Development, 19
Ji‐Quan Liu, T. Dairi, N. Itoh, M. Kataoka, Sakayu Shimizu, Hideaki Yamada (1998)
A Novel Metal-activated Pyridoxal Enzyme with a Unique Primary Structure, Low Specificity d-Threonine Aldolase fromArthrobacter sp. Strain DK-38The Journal of Biological Chemistry, 273
Nina Dückers, K. Baer, Sabine Simon, H. Gröger, W. Hummel (2010)
Threonine aldolases—screening, properties and applications in the synthesis of non-proteinogenic β-hydroxy-α-amino acidsApplied Microbiology and Biotechnology, 88
M. Kataoka, T. Miyakawa, S. Shimizu, M. Tanokura (2016)
Enzymes useful for chiral compound synthesis: structural biology, directed evolution, and protein engineering for industrial useApplied Microbiology and Biotechnology, 100
J. Liu, M. Odani, T. Dairi, N. Itoh, S. Shimizu, H. Yamada (1999)
A new route to l-threo-3-[4-(methylthio)phenylserine], a key intermediate for the synthesis of antibiotics: recombinant low-specificity d-threonine aldolase-catalyzed stereospecific resolutionApplied Microbiology and Biotechnology, 51
d‐Threonine aldolase from the green alga Chlamydomonas reinhardtii (CrDTA) catalyzes the interconversion of several β‐hydroxy‐d‐amino acids (e.g.d‐threonine) and glycine plus the corresponding aldehydes. Recombinant CrDTA was overexpressed in Escherichia coli and purified to homogeneity; it was subsequently crystallized using the hanging‐drop vapour‐diffusion method at 295 K. Data were collected and processed at 1.85 Å resolution. Analysis of the diffraction pattern showed that the crystal belonged to space group P1, with unit‐cell parameters a = 64.79, b = 74.10, c = 89.94 Å, α = 77.07, β = 69.34, γ = 71.93°. The asymmetric unit contained four molecules of CrDTA. The Matthews coefficient was calculated to be 2.12 Å3 Da−1 and the solvent content was 41.9%.
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2017
Keywords: ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.