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A galactose‐specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belonged to space group P1, with unit‐cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X‐ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image‐plate detector system mounted on a rotating‐anode X‐ray (Cu Kα) generator. Molecular replacement using legume‐lectin coordinates as a search model gave a tetrameric structure.
Acta Crystallographica Section F – Wiley
Published: Jun 1, 2011
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