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Crystallization and preliminary X‐ray studies of a galactose‐specific lectin from the seeds of Spatholobus parviflorus

Crystallization and preliminary X‐ray studies of a galactose‐specific lectin from the seeds of... A galactose‐specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belonged to space group P1, with unit‐cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X‐ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image‐plate detector system mounted on a rotating‐anode X‐ray (Cu Kα) generator. Molecular replacement using legume‐lectin coordinates as a search model gave a tetrameric structure. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and preliminary X‐ray studies of a galactose‐specific lectin from the seeds of Spatholobus parviflorus

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References (20)

Publisher
Wiley
Copyright
International Union of Crystallography, 2011
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S174430911101387X
pmid
21636916
Publisher site
See Article on Publisher Site

Abstract

A galactose‐specific seed lectin was purified from the legume Spatholobus parviflorus and crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belonged to space group P1, with unit‐cell parameters a = 60.998, b = 60.792, c = 78.179 Å, α = 101.32, β = 91.38, γ = 104.32°. X‐ray diffraction data were collected under cryoconditions (100 K) to a resolution of 2.04 Å using a MAR image‐plate detector system mounted on a rotating‐anode X‐ray (Cu Kα) generator. Molecular replacement using legume‐lectin coordinates as a search model gave a tetrameric structure.

Journal

Acta Crystallographica Section FWiley

Published: Jun 1, 2011

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