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References (13)
-
Mingdong Huang, J. Weissman, S. Béraud-Dufour, P. Luan, Chenqian Wang, Wei Chen, M. Aridor, I. Wilson, W. Balch (2001)
Crystal structure of Sar1-GDP at 1.7 Å resolution and the role of the NH2 terminus in ER exportThe Journal of Cell Biology, 155
-
T. Konishi, Keitaro Kato, T. Araki, K. Shiraki, M. Takagi, Y. Tamaru (2005)
A new class of glutathione S-transferase from the hepatopancreas of the red sea bream Pagrus major.The Biochemical journal, 388 Pt 1
-
Chunxin Fan, Shicui Zhang, Zhenhui Liu, Lei Li, J. Luan, G. Saren (2007)
Identification and expression of a novel class of glutathione-S-transferase from amphioxus Branchiostoma belcheri with implications to the origin of vertebrate liver.The international journal of biochemistry & cell biology, 39 2
-
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
-
A. Oakley (2005)
Glutathione transferases: new functions.Current opinion in structural biology, 15 6
-
A. Oakley, M. Bello, M. Nuccetelli, A. Mazzetti, M. Parker (1999)
The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site).Journal of molecular biology, 291 4
-
Rungrutai Udomsinprasert, Saengtong Pongjaroenkit, J. Wongsantichon, A. Oakley, L. Prapanthadara, M. Wilce, A. Ketterman (2005)
Identification, characterization and structure of a new Delta class glutathione transferase isoenzyme.The Biochemical journal, 388 Pt 3
-
Z. Otwinowski, W. Minor (1997)
[20] Processing of X-ray diffraction data collected in oscillation mode.Methods in enzymology, 276
-
R. Cardoso, D. Daniels, C. Bruns, J. Tainer (2003)
Characterization of the electrophile binding site and substrate binding mode of the 26‐kDa glutathione S‐transferase from Schistosoma japonicumProteins: Structure, 51
-
(1997)
Protein Expr -
B. Coles, B. Ketterer (1990)
The role of glutathione and glutathione transferases in chemical carcinogenesis.Critical reviews in biochemistry and molecular biology, 25 1
-
H. Park, I. Ahn, H. Choi, S. Pyo, H. Lee (2007)
Cloning, expression and characterization of metallothionein from the Antarctic clam Laternula elliptica.Protein expression and purification, 52 1
-
(1997)
Chem
- Publisher
- Wiley
- Copyright
- International Union of Crystallography, 2008
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309108034003
- pmid
- 19052367
- Publisher site
- See Article on Publisher Site
Abstract
Glutathione S‐transferases are involved in phase II detoxification processes and catalyze the nucleophilic attack of the tripeptide glutathione on a wide range of endobiotic and xenobiotic electrophilic substrates. The ρ‐class glutathione S‐transferase from Laternula elliptica was overexpressed in Escherichia coli, purified and crystallized with two substrates: glutathione and 1‐chloro‐2,4‐dinitrobenzene (CDNB). Diffraction data were collected to 2.20 Å resolution for the glutathione‐complex crystals and to 2.00 Å resolution for the CDNB‐complex crystals using a synchrotron‐radiation source. Both crystals belonged to the C‐centred monoclinic space group C2. The unit‐cell parameters for the CDNB‐complex crystals were a = 89.66, b = 59.27, c = 55.45 Å, β = 124.52°. The asymmetric unit contained one molecule, with a corresponding VM of 2.36 Å3 Da−1 and a solvent content of 47.8%.
Journal
Acta Crystallographica Section F – Wiley
Published: Dec 1, 2008