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Crystallization and preliminary X‐ray crystallographic analysis of the short‐chain dehydrogenase/reductase‐type DDB_G0291732 protein from Dictyostelium discoideum

Crystallization and preliminary X‐ray crystallographic analysis of the short‐chain... The DDB_G0291732 gene product from Dictyostelium discoideum, which is a NmrA‐like protein that belongs to the short‐chain dehydrogenase/reductase superfamily but shows deviations in conserved sequence regions, has been crystallized by the hanging‐drop vapour‐diffusion method at 295 K. A 1.65 Å resolution data set was collected using synchrotron radiation. The crystals of DDB_G0291732 protein belonged to space group P21, with unit‐cell parameters a = 38.5, b = 63.7, c = 56.0 Å, β = 91.7°. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 38.1%. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and preliminary X‐ray crystallographic analysis of the short‐chain dehydrogenase/reductase‐type DDB_G0291732 protein from Dictyostelium discoideum

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References (1)

Publisher
Wiley
Copyright
International Union of Crystallography, 2011
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309110046932
pmid
21206035
Publisher site
See Article on Publisher Site

Abstract

The DDB_G0291732 gene product from Dictyostelium discoideum, which is a NmrA‐like protein that belongs to the short‐chain dehydrogenase/reductase superfamily but shows deviations in conserved sequence regions, has been crystallized by the hanging‐drop vapour‐diffusion method at 295 K. A 1.65 Å resolution data set was collected using synchrotron radiation. The crystals of DDB_G0291732 protein belonged to space group P21, with unit‐cell parameters a = 38.5, b = 63.7, c = 56.0 Å, β = 91.7°. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 38.1%.

Journal

Acta Crystallographica Section F – Wiley

Published: Jan 1, 2011

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