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(1981)
Annu
- Publisher
- Wiley
- Copyright
- Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309106001448
- pmid
- 16511291
- Publisher site
- See Article on Publisher Site
Abstract
The galactose‐specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging‐drop vapour‐diffusion technique. The crystals belong to space group P1, with unit‐cell parameters a = 73.99, b = 84.13, c = 93.15 Å, α = 89.92, β = 76.01, γ = 76.99°. X‐ray diffraction data to a resolution of 3.0 Å have been collected under cryoconditions (100 K) using a MAR imaging‐plate detector system mounted on a rotating‐anode X‐ray generator. Molecular‐replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose‐specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.
Journal
Acta Crystallographica Section F – Wiley
Published: Feb 1, 2006