Access the full text.
Sign up today, get DeepDyve free for 14 days.
X. Saulquin, C. Ibisch, M. Peyrat, E. Scotet, M. Hourmant, H. Vié, M. Bonneville, E. Houssaint (2000)
A global appraisal of immunodominant CD8 T cell responses to Epstein‐Barr virus and cytomegalovirus by bulk screeningEuropean Journal of Immunology, 30
M. Wills, A. Carmichael, Kim Mynard, X. Jin, M. Weekes, B. Plachter, J. Sissons (1996)
The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTLJournal of Virology, 70
L. Borysiewicz, Julian Hickling, S. Graham, J Sinclair, M. Cranage, Geoffrey 'and, J. Sissons (1988)
Human cytomegalovirus-specific cytotoxic T cells. Relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia virusesThe Journal of Experimental Medicine, 168
S. Gras, X. Saulquin, J. Reiser, E. Debeaupuis, K. Echasserieau, A. Kissenpfennig, F. Legoux, A. Chouquet, Madalen Gorrec, P. Machillot, B. Neveu, N. Thielens, B. Malissen, M. Bonneville, D. Housset (2009)
Structural Bases for the Affinity-Driven Selection of a Public TCR against a Dominant Human Cytomegalovirus Epitope1The Journal of Immunology, 183
S. Dunn, P. Rizkallah, Emma Baston, T. Mahon, B. Cameron, R. Moysey, F. Gao, Malkit Sami, J. Boulter, Yi Li, B. Jakobsen (2006)
Directed evolution of human T cell receptor CDR2 residues by phage display dramatically enhances affinity for cognate peptide‐MHC without increasing apparent cross‐reactivityProtein Science, 15
D. Housset, G. Mazza, C. Grégoire, C. Piras, B. Malissen, J. Fontecilla-Camps (1997)
The three‐dimensional structure of a T‐cell antigen receptor VαVβ heterodimer reveals a novel arrangement of the Vβ domainThe EMBO Journal, 16
The NLV peptide is represented by violet sticks, the HLA-A2 1 helix in gold and the 2 helix in silver
Malkit Sami, P. Rizkallah, S. Dunn, P. Molloy, R. Moysey, A. Vuidepot, Emma Baston, P. Todorov, Yi Li, F. Gao, J. Boulter, B. Jakobsen (2007)
Crystal structures of high affinity human T-cell receptors bound to peptide major histocompatibility complex reveal native diagonal binding geometry.Protein engineering, design & selection : PEDS, 20 8
D. Diamond, Joanne York, Ji-Yao Sun, Christine Wright, Stephen Forman (1997)
Development of a candidate HLA A*0201 restricted peptide-based vaccine against human cytomegalovirus infection.Blood, 90 5
Superposition was performed on the 12 HLA-A2 domain. (a) Overall view of the superimposition, (b) closer view of the TCR-NLV-A2 interface
M. Engstrand, C. Tournay, M. Peyrat, B. Eriksson, J. Wadström, B. Wirgart, F. Romagné, M. Bonneville, T. Tötterman, O. Korsgren (2000)
Characterization of CMVpp65-specific CD8+ T lymphocytes using MHC tetramers in kidney transplant patients and healthy participants.Transplantation, 69 11
J. Sissons, M. Bain, Mark Wills (2002)
Latency and reactivation of human cytomegalovirus.The Journal of infection, 44 2
BY Navaza (1994)
AMoRe: an automated package for molecular replacementActa Crystallographica Section A, 50
J. Boulter, M. Glick, P. Todorov, Emma Baston, Malkit Sami, P. Rizkallah, B. Jakobsen (2003)
Stable, soluble T-cell receptor molecules for crystallization and therapeutics.Protein engineering, 16 9
K. Peggs, S. Verfuerth, A. Pizzey, J. Ainsworth, P. Moss, S. Mackinnon (2002)
Characterization of human cytomegalovirus peptide-specific CD8(+) T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells.Blood, 99 1
L. Trautmann, M. Rimbert, K. Echasserieau, X. Saulquin, B. Neveu, J. Déchanet, V. Cerundolo, M. Bonneville (2005)
Selection of T Cell Clones Expressing High-Affinity Public TCRs within Human Cytomegalovirus-Specific CD8 T Cell Responses1The Journal of Immunology, 175
(1994)
Acta Cryst
J. Archbold, W. Macdonald, S. Gras, L. Ely, John Miles, Melissa Bell, R. Brennan, T. Beddoe, M. Wilce, C. Clements, A. Purcell, J. McCluskey, S. Burrows, J. Rossjohn (2009)
Natural micropolymorphism in human leukocyte antigens provides a basis for genetic control of antigen recognitionThe Journal of Experimental Medicine, 206
E. McLaughlin‐Taylor, H. Pande, S. Forman, B. Tanamachi, Chengrong Li, J. Zaia, P. Greenberg, S. Riddell (1994)
Identification of the major late human cytomegalovirus matrix protein pp65 as a target antigen for CD8+ virus‐specific cytotoxic T lymphocytesJournal of Medical Virology, 43
W. Kabsch (1993)
Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constantsJournal of Applied Crystallography, 26
C. Grégoire, B. Malissen, G. Mazza (1996)
Characterization of T cell receptor single‐chain Fv fragments secreted by myeloma cellsEuropean Journal of Immunology, 26
(2001)
Nucleic Acids Res
S. Gras, S. Burrows, L. Kjer-Nielsen, C. Clements, Y. Liu, L. Sullivan, Melissa Bell, A. Brooks, A. Purcell, J. McCluskey, J. Rossjohn (2009)
The shaping of T cell receptor recognition by self-tolerance.Immunity, 30 2
The T‐cell response to human cytomegalovirus is characterized by a dramatic reduction of clonal diversity in patients undergoing chronic inflammation or immunodepression. In order to check whether all the selected high‐avidity T‐cell clones recognize the immunodominant pp65 peptide antigen pp65495–503 (NLVPMVATV) presented by the major histocompatibility complex (MHC) molecule HLA‐A2 in a similar manner, several public high‐affinity T‐cell receptors (TCRs) specific for the pp65495–503–HLA‐A2 complex have been investigated. Expression, purification and crystallization were performed and preliminary crystallographic data were collected to 4.7 Å resolution for the RA15 TCR in complex with the pp65495–503–HLA‐A2 complex. Comparison of the RA15–pp65495–503–HLA‐A2 complex molecular‐replacement solution with the structure of another high‐affinity pp65495–503–HLA‐A2‐specific TCR, RA14, shows a shared docking mode, indicating that the clonal focusing could be accompanied by the selection of a most favoured peptide‐readout mode. However, the position of the RA15 Vβ domain is significantly shifted, suggesting a different interatomic interaction network.
Acta Crystallographica Section F – Wiley
Published: Nov 1, 2009
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.