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References (16)
-
L. Stirling, K. Willcox, P. Ferguson, D. Newman (2009)
Kinetics and kinematics for translational motions in microgravity during parabolic flight.Aviation, space, and environmental medicine, 80 6
-
R. Deshaies (1999)
SCF and Cullin/Ring H2-based ubiquitin ligases.Annual review of cell and developmental biology, 15
-
C. Thaller, L. Weaver, G. Eichele, E. Wilson, R. Karlsson, J. Jansonius (1981)
Repeated seeding technique for growing large single crystals of proteins.Journal of molecular biology, 147 3
-
T. Mizushima, Y. Yoshida, Taichi Kumanomidou, Y. Hasegawa, A. Suzuki, T. Yamane, Keiji Tanaka (2007)
Structural basis for the selection of glycosylated substrates by SCFFbs1 ubiquitin ligaseProceedings of the National Academy of Sciences, 104
-
T. Mizushima, T. Hirao, Y. Yoshida, Soo Lee, T. Chiba, K. Iwai, Y. Yamaguchi, Koichi Kato, T. Tsukihara, Keiji Tanaka (2004)
Structural basis of sugar-recognizing ubiquitin ligaseNature Structural &Molecular Biology, 11
-
C. Pickart (2001)
Mechanisms underlying ubiquitination.Annual review of biochemistry, 70
-
Y. Yoshida, T. Chiba, F. Tokunaga, H. Kawasaki, K. Iwai, Toshiaki Suzuki, Yukishige Ito, K. Matsuoka, Minoru Yoshida, Keiji Tanaka, T. Tai (2002)
E3 ubiquitin ligase that recognizes sugar chainsNature, 418
-
Y. Yoshida (2007)
F-Box Proteins That Contain Sugar-Binding DomainsBioscience, Biotechnology, and Biochemistry, 71
-
H. Adachi, H. Matsumura, K. Takano, A. Niino, Tsuyoshi Inoue, Y. Mori, T. Sasaki (2003)
Two-Liquid Hanging-Drop Vapor-Diffusion Technique of Protein CrystallizationJapanese Journal of Applied Physics, 43
-
M. Adams (1983)
Preparation and Analysis of Protein CrystalsBiochemical Society Transactions, 11
-
P. Matthews (1968)
Epidemic Self-Injury in an Adolescent UnitInternational Journal of Social Psychiatry, 14
-
Z. Otwinowski, W. Minor (1997)
[20] Processing of X-ray diffraction data collected in oscillation mode.Methods in enzymology, 276
-
Y. Yoshida, Arisa Murakami, K. Iwai, Keiji Tanaka (2007)
A Neural-specific F-box Protein Fbs1 Functions as a Chaperone Suppressing Glycoprotein Aggregation*Journal of Biological Chemistry, 282
-
Jianping Jin, T. Cardozo, R. Lovering, S. Elledge, M. Pagano, J. Harper (2004)
Systematic analysis and nomenclature of mammalian F-box proteins.Genes & development, 18 21
-
Hershko (1998)
425Annu. Rev. Biochem., 67
-
A. Hershko, A. Ciechanover, A. Varshavsky (2000)
The ubiquitin systemNature Medicine, 6
- Publisher
- Wiley
- Copyright
- International Union of Crystallography, 2010
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309109050581
- pmid
- 20057081
- Publisher site
- See Article on Publisher Site
Abstract
F‐box proteins are the substrate‐recognition components of Skp1–Cullin1–F‐box protein–Rbx1 (SCF) ubiquitin ligase complexes. Fbs1, an F‐box protein, binds specifically to proteins modified with high‐mannose oligosaccharides. Fbg3, another F‐box protein, has 51% sequence identity to Fbs1. Although the residues that are necessary for binding to oligosaccharides are conserved between Fbs1 and Fbg3, Fbg3 does not bind glycoproteins. Skp1 and Fbg3 were co‐expressed in Escherichia coli and their complex was purified to homogeneity and crystallized. Microseeding combined with the sandwiched hanging‐drop technique improved the quality of the resulting crystals. The plate‐shaped crystals belonged to space group P212121, with unit‐cell parameters a = 34.1, b = 76.6, c = 193.9 Å and one molecule per asymmetric unit.
Journal
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2010