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P. Setlow (2007)
I will survive: DNA protection in bacterial spores.Trends in microbiology, 15 4
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
P. Setlow (2006)
Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicalsJournal of Applied Microbiology, 101
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
B. Setlow, Donxxu Sun, P. Setlow (1992)
Interaction between DNA and alpha/beta-type small, acid-soluble spore proteins: a new class of DNA-binding proteinJournal of Bacteriology, 174
C. Hayes, P. Setlow (1998)
Identification of Protein-Protein Contacts between α/β-Type Small, Acid-soluble Spore Proteins of Bacillus Species Bound to DNA*The Journal of Biological Chemistry, 273
C. Hayes, P. Setlow (2001)
An α/β-Type, Small, Acid-Soluble Spore Protein Which Has Very High Affinity for DNA Prevents Outgrowth ofBacillus subtilis SporesJournal of Bacteriology, 183
Jeffrey Kosman, P. Setlow (2003)
Effects of Carboxy-Terminal Modifications and pH on Binding of a Bacillus subtilis Small, Acid-Soluble Spore Protein to DNAJournal of Bacteriology, 185
C. Hayes, E. Alarcón-Hernández, P. Setlow (2001)
N-terminal Amino Acid Residues Mediate Protein-Protein Interactions between DNA-bound α/β-Type Small, Acid-soluble Spore Proteins from Bacillus Species*The Journal of Biological Chemistry, 276
T. Jones, J. Zou, S. Cowan, M. Kjeldgaard, Serge HAGgGE (1991)
Improved methods for building protein models in electron density maps and the location of errors in these models.Acta crystallographica. Section A, Foundations of crystallography, 47 ( Pt 2)
S. Doublié (1997)
[29] Preparation of selenomethionyl proteins for phase determination.Methods in enzymology, 276
N. Magill, Annie Cowan, M. Leyva-Vázquez, Martin Brown, D. Koppel, P. Setlow (1996)
Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus speciesJournal of Bacteriology, 178
C. Hayes, Zheng‐yu Peng, P. Setlow (2000)
Equilibrium and Kinetic Binding Interactions between DNA and a Group of Novel, Nonspecific DNA-binding Proteins from Spores ofBacillus and Clostridium Species*The Journal of Biological Chemistry, 275
W. Scott, John Finch, Richard Grenfell, Jan Fogg, Terry Smith, Michael Gait, Aaron Klug (1995)
Rapid crystallization of chemically synthesized hammerhead RNAs using a double screening procedure.Journal of molecular biology, 250 3
Z. Otwinowski, W. Minor (1997)
[20] Processing of X-ray diffraction data collected in oscillation mode.Methods in enzymology, 276
N. Magill, Annie Cowan, D. Koppel, P. Setlow (1994)
The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulationJournal of Bacteriology, 176
D. Frenkiel-Krispin, Rinat Sack, J. Englander, E. Shimoni, M. Eisenstein, E. Bullitt, Rachel Horowitz-Scherer, C. Hayes, P. Setlow, A. Minsky, S. Wolf (2004)
Structure of the DNA-SspC Complex: Implications for DNA Packaging, Protection, and Repair in Bacterial SporesJournal of Bacteriology, 186
An engineered variant of an α/β‐type small acid‐soluble spore protein (SASP) from Bacillus subtilis was crystallized in a complex with a ten‐base‐pair double‐stranded DNA by the hanging‐drop vapor‐diffusion method using ammonium sulfate as a precipitating agent. Crystals grew at 281 K using sodium cacodylate buffer pH 5.5 and these crystals diffracted X‐rays to beyond 2.4 Å resolution using synchrotron radiation. The crystallized complex contains two or three SASP molecules bound to one DNA molecule. The crystals belong to the hexagonal space group P6122 or P6522, with unit‐cell parameters a = b = 87.0, c = 145.4 Å, α = β = 90.0, γ = 120.0°. Diffraction data were 96.6% complete to 2.4 Å resolution, with an Rsym of 8.5%. Structure solution by the multiwavelength/single‐wavelength anomalous dispersion method using isomorphous crystals of selenomethionine‐labeled protein is in progress.
Acta Crystallographica Section F – Wiley
Published: Jun 1, 2007
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