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Crystallization and preliminary X‐ray analysis of pyridoxine 4‐oxidase, the first enzyme in pyridoxine degradation pathway I

Crystallization and preliminary X‐ray analysis of pyridoxine 4‐oxidase, the first enzyme in... Vitamin B6‐degradation pathway I has recently been identified in Mesorhizobium loti MAFF303099. Pyridoxine 4‐oxidase, an FAD‐dependent enzyme, is the first enzyme in this pathway and catalyzes the irreversible oxidation of pyridoxine to pyridoxal. The enzyme was overexpressed in Escherichia coli with a His6 tag and purified. The recombinant enzyme was crystallized at 277 K by the sitting‐drop vapour‐diffusion method using PEG 4000 as the precipitant. The crystal, which belonged to space group P212121 with unit‐cell parameters a = 62.38, b = 79.44, c = 136.43 Å, diffracted to 2.2 Å resolution. The calculated VM value (3.19 Å3 Da–1) suggested that the asymmetric unit contained one molecule. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and preliminary X‐ray analysis of pyridoxine 4‐oxidase, the first enzyme in pyridoxine degradation pathway I

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References (17)

Publisher
Wiley
Copyright
International Union of Crystallography, 2012
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309111048688
pmid
22232175
Publisher site
See Article on Publisher Site

Abstract

Vitamin B6‐degradation pathway I has recently been identified in Mesorhizobium loti MAFF303099. Pyridoxine 4‐oxidase, an FAD‐dependent enzyme, is the first enzyme in this pathway and catalyzes the irreversible oxidation of pyridoxine to pyridoxal. The enzyme was overexpressed in Escherichia coli with a His6 tag and purified. The recombinant enzyme was crystallized at 277 K by the sitting‐drop vapour‐diffusion method using PEG 4000 as the precipitant. The crystal, which belonged to space group P212121 with unit‐cell parameters a = 62.38, b = 79.44, c = 136.43 Å, diffracted to 2.2 Å resolution. The calculated VM value (3.19 Å3 Da–1) suggested that the asymmetric unit contained one molecule.

Journal

Acta Crystallographica Section FWiley

Published: Jan 1, 2012

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