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Vitamin B6‐degradation pathway I has recently been identified in Mesorhizobium loti MAFF303099. Pyridoxine 4‐oxidase, an FAD‐dependent enzyme, is the first enzyme in this pathway and catalyzes the irreversible oxidation of pyridoxine to pyridoxal. The enzyme was overexpressed in Escherichia coli with a His6 tag and purified. The recombinant enzyme was crystallized at 277 K by the sitting‐drop vapour‐diffusion method using PEG 4000 as the precipitant. The crystal, which belonged to space group P212121 with unit‐cell parameters a = 62.38, b = 79.44, c = 136.43 Å, diffracted to 2.2 Å resolution. The calculated VM value (3.19 Å3 Da–1) suggested that the asymmetric unit contained one molecule.
Acta Crystallographica Section F – Wiley
Published: Jan 1, 2012
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