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Crystallization and preliminary X‐ray analysis of Aspergillus oryzae catechol oxidase

Crystallization and preliminary X‐ray analysis of Aspergillus oryzae catechol oxidase Catechol oxidase is an enzyme that catalyzes the oxidation of o‐diphenols to the corresponding o‐quinones. It is a copper‐containing enzyme with a binuclear copper active site. Here, the crystallization and multiple‐wavelength anomalous dispersion data collection of catechol oxidase from the mould fungus Aspergillus oryzae are described. During the purification, three forms of the enzyme (39.3, 40.5 and 44.3 kDa) were obtained. A mixture of these three forms was initially crystallized and gave crystals that diffracted to 2.5 Å resolution and belonged to space group P3221, with unit‐cell parameters a = b = 118.9, c = 84.5 Å, α = β = 90, γ = 120°. A preparation containing only the shorter form (39.3 kDa) produced crystals that diffracted to 2.9 Å resolution and belonged to space group P212121, with unit‐cell parameters a = 51.8, b = 95.3, c = 139.5 Å, α = β = γ = 90°. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and preliminary X‐ray analysis of Aspergillus oryzae catechol oxidase

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References (18)

Publisher
Wiley
Copyright
International Union of Crystallography, 2011
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309111010141
pmid
21636908
Publisher site
See Article on Publisher Site

Abstract

Catechol oxidase is an enzyme that catalyzes the oxidation of o‐diphenols to the corresponding o‐quinones. It is a copper‐containing enzyme with a binuclear copper active site. Here, the crystallization and multiple‐wavelength anomalous dispersion data collection of catechol oxidase from the mould fungus Aspergillus oryzae are described. During the purification, three forms of the enzyme (39.3, 40.5 and 44.3 kDa) were obtained. A mixture of these three forms was initially crystallized and gave crystals that diffracted to 2.5 Å resolution and belonged to space group P3221, with unit‐cell parameters a = b = 118.9, c = 84.5 Å, α = β = 90, γ = 120°. A preparation containing only the shorter form (39.3 kDa) produced crystals that diffracted to 2.9 Å resolution and belonged to space group P212121, with unit‐cell parameters a = 51.8, b = 95.3, c = 139.5 Å, α = β = γ = 90°.

Journal

Acta Crystallographica Section FWiley

Published: Jun 1, 2011

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