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The bacteriophage λ O protein binds to the λ replication origin (oriλ) and serves as the primary replication initiator for the viral genome. The binding energy derived from the binding of O to oriλ is thought to help drive DNA opening to facilitate initiation of DNA replication. Detailed understanding of this process is severely limited by the lack of high‐resolution structures of O protein or of any lambdoid phage‐encoded paralogs either with or without DNA. The production of crystals of the origin‐binding domain of λ O that diffract to 2.5 Å is reported. Anomalous dispersion methods will be used to solve this structure.
Acta Crystallographica Section F – Wiley
Published: Jun 1, 2007
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