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Crystallization and preliminary crystallographic analysis of d ‐alanine‐ d ‐alanine ligase from Streptococcus mutans

Crystallization and preliminary crystallographic analysis of d ‐alanine‐ d ‐alanine ligase from... d‐Alanine‐d‐alanine ligase is encoded by the gene ddl (SMU_599) in Streptococcus mutans. This ligase plays a very important role in cell‐wall biosynthesis and may be a potential target for drug design. To study the structure and function of this ligase, the gene ddl was amplified from S. mutans genomic DNA and cloned into the expression vector pET28a. The protein was expressed in soluble form in Escherichia coli strain BL21 (DE3). Homogeneous protein was obtained using a two‐step procedure consisting of Ni2+‐chelating and size‐exclusion chromatography. Purified protein was crystallized and the cube‐shaped crystal diffracted to 2.4 Å. The crystal belongs to space group P3121 or P3221, with unit‐cell parameters a = b = 79.50, c = 108.97 Å. There is one molecule per asymmetric unit. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and preliminary crystallographic analysis of d ‐alanine‐ d ‐alanine ligase from Streptococcus mutans

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References (6)

Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309107040298
pmid
17768361
Publisher site
See Article on Publisher Site

Abstract

d‐Alanine‐d‐alanine ligase is encoded by the gene ddl (SMU_599) in Streptococcus mutans. This ligase plays a very important role in cell‐wall biosynthesis and may be a potential target for drug design. To study the structure and function of this ligase, the gene ddl was amplified from S. mutans genomic DNA and cloned into the expression vector pET28a. The protein was expressed in soluble form in Escherichia coli strain BL21 (DE3). Homogeneous protein was obtained using a two‐step procedure consisting of Ni2+‐chelating and size‐exclusion chromatography. Purified protein was crystallized and the cube‐shaped crystal diffracted to 2.4 Å. The crystal belongs to space group P3121 or P3221, with unit‐cell parameters a = b = 79.50, c = 108.97 Å. There is one molecule per asymmetric unit.

Journal

Acta Crystallographica Section FWiley

Published: Sep 1, 2007

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