Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Saiki, D. Gelfand, S. Stoffel, S. Scharf, R. Higuchi, G. Horn, K. Mullis, H. Erlich (1988)
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.Science, 239 4839
Jun Lee, Y. Na, Hye‐Eun Song, Dooil Kim, B. Park, S. Rho, Y. Im, Mun‐kyoung Kim, G. Kang, Dae‐Sil Lee, S. Eom (2006)
Crystal structure of the apo form of D‐alanine: D‐alanine ligase (Ddl) from Thermus caldophilus: A basis for the substrate‐induced conformational changesProteins: Structure, 64
F. Neuhaus (1962)
The enzymatic synthesis of D-alanyl-D-alanine. I. Purification and properties of D-alanyl-D-alanine synthetase.The Journal of biological chemistry, 237
B. Matthews (1968)
Solvent content of protein crystals.Journal of molecular biology, 33 2
(2006)
Proc
C. Fan, I. Park, C. Walsh, J. Knox (1997)
D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant.Biochemistry, 36 9
d‐Alanine‐d‐alanine ligase is encoded by the gene ddl (SMU_599) in Streptococcus mutans. This ligase plays a very important role in cell‐wall biosynthesis and may be a potential target for drug design. To study the structure and function of this ligase, the gene ddl was amplified from S. mutans genomic DNA and cloned into the expression vector pET28a. The protein was expressed in soluble form in Escherichia coli strain BL21 (DE3). Homogeneous protein was obtained using a two‐step procedure consisting of Ni2+‐chelating and size‐exclusion chromatography. Purified protein was crystallized and the cube‐shaped crystal diffracted to 2.4 Å. The crystal belongs to space group P3121 or P3221, with unit‐cell parameters a = b = 79.50, c = 108.97 Å. There is one molecule per asymmetric unit.
Acta Crystallographica Section F – Wiley
Published: Sep 1, 2007
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.