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Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate

Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate Bacterial lipases that are secreted via the type II secretion pathway require a lipase‐specific foldase in order to obtain their native and biologically active conformation in the periplasmic space. The lipase–foldase complex from Burkholderia glumae (319 and 333 residues, respectively) was crystallized in two crystal forms. One crystal form belongs to space group P3121 (P3221), with unit‐cell parameters a = b = 122.3, c = 98.2 Å. A procedure is presented which improved the diffraction of these crystals from ∼5 to 2.95 Å. For the second crystal form, which belonged to space group C2 with unit‐cell parameters a = 183.0, b = 75.7, c = 116.6 Å, X‐ray data were collected to 1.85 Å. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate

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Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309105023055
pmid
16511160
Publisher site
See Article on Publisher Site

Abstract

Bacterial lipases that are secreted via the type II secretion pathway require a lipase‐specific foldase in order to obtain their native and biologically active conformation in the periplasmic space. The lipase–foldase complex from Burkholderia glumae (319 and 333 residues, respectively) was crystallized in two crystal forms. One crystal form belongs to space group P3121 (P3221), with unit‐cell parameters a = b = 122.3, c = 98.2 Å. A procedure is presented which improved the diffraction of these crystals from ∼5 to 2.95 Å. For the second crystal form, which belonged to space group C2 with unit‐cell parameters a = 183.0, b = 75.7, c = 116.6 Å, X‐ray data were collected to 1.85 Å.

Journal

Acta Crystallographica Section FWiley

Published: Aug 1, 2005

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