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Crystal structure of an IclR homologue from Microbacterium sp. strain HM58‐2

Crystal structure of an IclR homologue from Microbacterium sp. strain HM58‐2 The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one‐component signal transduction system, which shares the common motif of a helix–turn–helix (HTH)‐type DNA‐binding domain (DBD) connected to a substrate‐binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi‐IclR) from Microbacterium sp. strain HM58‐2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi‐IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step. Native single‐wavelength anomalous diffraction (SAD) experiments were performed in combination with molecular replacement. CRANK2 from the CCP4 suite successfully phased and modelled the complete structure of a homotetramer composed of 1000 residues in an asymmetric unit, and the model was refined to 2.1 Å resolution. The overall structure of Mi‐IclR shared the same domain combination as other known IclR structures, but the relative geometry between the DBD and SBD differs. Accordingly, the geometry of the Mi‐IclR tetramer was unique: the putative substrate‐binding site in each subunit is accessible from the outside of the tetramer, as opposed to buried inside as in the previously known IclR structures. These differences in the domain geometry may contribute to the transcriptional regulation of IclRs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Crystal structure of an IclR homologue from Microbacterium sp. strain HM58‐2

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References (56)

Publisher
Wiley
Copyright
Copyright © 2017 Wiley Subscription Services
ISSN
2053-230X
eISSN
2053-230X
DOI
10.1107/S2053230X16019208
pmid
28045389
Publisher site
See Article on Publisher Site

Abstract

The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one‐component signal transduction system, which shares the common motif of a helix–turn–helix (HTH)‐type DNA‐binding domain (DBD) connected to a substrate‐binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi‐IclR) from Microbacterium sp. strain HM58‐2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi‐IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step. Native single‐wavelength anomalous diffraction (SAD) experiments were performed in combination with molecular replacement. CRANK2 from the CCP4 suite successfully phased and modelled the complete structure of a homotetramer composed of 1000 residues in an asymmetric unit, and the model was refined to 2.1 Å resolution. The overall structure of Mi‐IclR shared the same domain combination as other known IclR structures, but the relative geometry between the DBD and SBD differs. Accordingly, the geometry of the Mi‐IclR tetramer was unique: the putative substrate‐binding site in each subunit is accessible from the outside of the tetramer, as opposed to buried inside as in the previously known IclR structures. These differences in the domain geometry may contribute to the transcriptional regulation of IclRs.

Journal

Acta Crystallographica Section FWiley

Published: Jan 1, 2017

Keywords: ; ; ; ;

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