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Controlling Localization and Excretion of Nanoparticles by Click Modification of the Surface Chemical Structures inside Living Cells

Controlling Localization and Excretion of Nanoparticles by Click Modification of the Surface... What prompted you to investigate this topic/problem? As a member of the “Kyoto Environmental Nanotechnology Cluster”, our group evaluated the cytotoxic effects of various engineered nanoparticles, prior to commencing work on the current project. During this time, we were not fully aware of the mechanism underlying the interactions between engineered nanoparticles and intracellular biomolecules as well as the reason for the cytotoxic effects of these nanoparticles. Nowadays, the application of such nanoparticles as vehicles for drug delivery is widely envisioned. We believe that understanding the interactions between nanoparticles and biomolecules that affect their intracellular dynamics is essential for developing safer drug delivery systems. 1 Scheme Takeo Ito 2 Scheme Takuma Nakamura 3 Scheme Eriko Kusaka 4 Scheme Ryohsuke Kurihara 5 Scheme Kazuhito Tanabe 6 Scheme What is the most significant result of this study? Previous studies have shown that chemical modification of the surface of nanoparticles can enhance their internalization within living cells. However, very little is known about the effect of the surface chemical structures on the excretion of nanoparticles from the cells. Our results have shown that in situ modification of the surface with phospholipids through click chemistry induces translocation of the intracellular nanoparticles to the surface of the plasma membrane. Furthermore, the kinetics and extent of elimination of the nanoparticles from the cells are enhanced. The current strategy provides a promising new method to control the dynamic behavior of the nanoparticles inside living cells and this approach could be applied to novel drug delivery systems. How did each team member contribute to the work? Our group is made up of researchers with diverse backgrounds, such as organic chemistry, physical chemistry, and biology. Takeo Ito and Eriko Kusaka started this project and demonstrated that click translocation could work. Takuma Nakamura, Ryohsuke Kurihara, and Kazuhito Tanabe further developed the method by synthesizing clickable silica nanoparticles. We are always respectful of each other’s experience and knowledge, which we believe is the key to our success. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png ChemPlusChem Wiley

Controlling Localization and Excretion of Nanoparticles by Click Modification of the Surface Chemical Structures inside Living Cells

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Publisher
Wiley
Copyright
Copyright © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
2192-6506
eISSN
2192-6506
DOI
10.1002/cplu.201500124
Publisher site
See Article on Publisher Site

Abstract

What prompted you to investigate this topic/problem? As a member of the “Kyoto Environmental Nanotechnology Cluster”, our group evaluated the cytotoxic effects of various engineered nanoparticles, prior to commencing work on the current project. During this time, we were not fully aware of the mechanism underlying the interactions between engineered nanoparticles and intracellular biomolecules as well as the reason for the cytotoxic effects of these nanoparticles. Nowadays, the application of such nanoparticles as vehicles for drug delivery is widely envisioned. We believe that understanding the interactions between nanoparticles and biomolecules that affect their intracellular dynamics is essential for developing safer drug delivery systems. 1 Scheme Takeo Ito 2 Scheme Takuma Nakamura 3 Scheme Eriko Kusaka 4 Scheme Ryohsuke Kurihara 5 Scheme Kazuhito Tanabe 6 Scheme What is the most significant result of this study? Previous studies have shown that chemical modification of the surface of nanoparticles can enhance their internalization within living cells. However, very little is known about the effect of the surface chemical structures on the excretion of nanoparticles from the cells. Our results have shown that in situ modification of the surface with phospholipids through click chemistry induces translocation of the intracellular nanoparticles to the surface of the plasma membrane. Furthermore, the kinetics and extent of elimination of the nanoparticles from the cells are enhanced. The current strategy provides a promising new method to control the dynamic behavior of the nanoparticles inside living cells and this approach could be applied to novel drug delivery systems. How did each team member contribute to the work? Our group is made up of researchers with diverse backgrounds, such as organic chemistry, physical chemistry, and biology. Takeo Ito and Eriko Kusaka started this project and demonstrated that click translocation could work. Takuma Nakamura, Ryohsuke Kurihara, and Kazuhito Tanabe further developed the method by synthesizing clickable silica nanoparticles. We are always respectful of each other’s experience and knowledge, which we believe is the key to our success.

Journal

ChemPlusChem – Wiley

Published: May 1, 2015

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