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Complement C3 inhibitor Cp40 attenuates xenoreactions in pig hearts perfused with human blood

Complement C3 inhibitor Cp40 attenuates xenoreactions in pig hearts perfused with human blood Maximilian University, Munich, Germany Background: The complement system plays a crucial role in acute xenogeneic reactions Department of Clinical Pathobiochemistry, Medical after cardiac transplantation. We used an ex vivo perfusion model to investigate the Faculty, Technische Universität Dresden, effect of Cp40, a compstatin analog and potent inhibitor of complement at the level of C3. Dresden, Germany Walter-Brendel-Centre, Ludwig Maximilian Methods: Fifteen wild- type pig hearts were explanted, cardiopleged, and reperfused University, Munich, Germany ex vivo after 150 minutes of cold ischemia. Hearts were challenged in a biventricular Department of Pathology and Laboratory working heart mode to evaluate cardiac perfusion and function. In the treatment Medicine, University of Pennsylvania, Pennsylvania, PA, USA group (n=5), the complement cascade was blocked at the level of C3 using Cp40, using Institute of Laboratory Medicine of Ludwig diluted human blood. Untreated human and porcine blood was used for controls. Maximilian University, Munich, Germany Results: Throughout the perfusion, C3 activation was inhibited when Cp40 was used Chair of Livestock Biotechnology, School (mean of all time points: 1.11 ± 0.34% vs 3.12 ± 0.48% control activation; P<.01). of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany Compared to xenoperfused controls, the cardiac index improved significantly http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

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References (43)

Publisher
Wiley
Copyright
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/xen.12262
pmid
27677785
Publisher site
See Article on Publisher Site

Abstract

Maximilian University, Munich, Germany Background: The complement system plays a crucial role in acute xenogeneic reactions Department of Clinical Pathobiochemistry, Medical after cardiac transplantation. We used an ex vivo perfusion model to investigate the Faculty, Technische Universität Dresden, effect of Cp40, a compstatin analog and potent inhibitor of complement at the level of C3. Dresden, Germany Walter-Brendel-Centre, Ludwig Maximilian Methods: Fifteen wild- type pig hearts were explanted, cardiopleged, and reperfused University, Munich, Germany ex vivo after 150 minutes of cold ischemia. Hearts were challenged in a biventricular Department of Pathology and Laboratory working heart mode to evaluate cardiac perfusion and function. In the treatment Medicine, University of Pennsylvania, Pennsylvania, PA, USA group (n=5), the complement cascade was blocked at the level of C3 using Cp40, using Institute of Laboratory Medicine of Ludwig diluted human blood. Untreated human and porcine blood was used for controls. Maximilian University, Munich, Germany Results: Throughout the perfusion, C3 activation was inhibited when Cp40 was used Chair of Livestock Biotechnology, School (mean of all time points: 1.11 ± 0.34% vs 3.12 ± 0.48% control activation; P<.01). of Life Sciences Weihenstephan, Technical University of Munich, Munich, Germany Compared to xenoperfused controls, the cardiac index improved significantly

Journal

XenotransplantationWiley

Published: Jan 1, 2017

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