Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/cloning-purification-and-preliminary-crystallographic-analysis-of-the-Ti721LbcFl
References (26)
-
Jacqueline Tan, U. Jakob, J. Bardwell (2002)
Overexpression of Two Different GTPases Rescues a Null Mutation in a Heat-Induced rRNA MethyltransferaseJournal of Bacteriology, 184
-
T. Morimoto, P. Loh, T. Hirai, K. Asai, Kazuo Kobayashi, S. Moriya, N. Ogasawara (2002)
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.Microbiology, 148 Pt 11
-
C. Knudsen, Hans-Joachim Wieden, M. Rodnina (2001)
The Importance of Structural Transitions of the Switch II Region for the Functions of Elongation Factor Tu on the Ribosome*The Journal of Biological Chemistry, 276
-
E. Miadoková (1977)
GeneticsBiologia Plantarum, 19
-
(1991)
Nature -
I. Vetter, A. Wittinghofer (2001)
The Guanine Nucleotide-Binding Switch in Three DimensionsScience, 294
-
H. Bourne, D. Sanders, F. McCormick (1991)
The GTPase superfamily: conserved structure and molecular mechanismNature, 349
-
(2006)
Acta Cryst -
(2001)
Mol. Microbiol -
E. Greisheimer (1955)
The proteins.Journal of the American Medical Women's Association, 10 5
-
YphC–GDP complex 437 Table -
Detlef Leipe, Y. Wolf, E. Koonin, L. Aravind (2002)
Classification and evolution of P-loop GTPases and related ATPases.Journal of molecular biology, 317 1
-
Jihwan Hwang, M. Inouye (2001)
An Essential GTPase, Der, Containing Double GTP-binding Domains from Escherichia coli and Thermotoga maritima*The Journal of Biological Chemistry, 276
-
(2001)
Science crystallization communications -
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
-
(1992)
Jnt CCP 4 / ESF – EACBM Newsl -
V. Robinson, Jihwan Hwang, E. Fox, M. Inouye, Ann Stock (2002)
Domain arrangement of Der, a switch protein containing two GTPase domains.Structure, 10 12
-
(2002)
J. Mol. Biol -
S. Eykyn (1950)
MicrobiologyThe Yale Journal of Biology and Medicine, 22
-
Data set Peak ( 1 ) Inflection ( 2 ) Remote ( 3 ) Wavelength (A ˚ ) 0.9794 0.9797 1.0094 Resolution (A ˚ ) 30–2 -
(2001)
J. Biol. Chem -
(1994)
Collaborative Computational Project, Number Acta Cryst. D50 -
Data-collection statistics -
Catherine Caldon, Pauline Yoong, P. March (2001)
Evolution of a molecular switch: universal bacterial GTPases regulate ribosome functionMolecular Microbiology, 41
-
I. Mehr, C. Long, C. Serkin, H. Seifert (2000)
A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation.Genetics, 154 2
-
(2002)
J. Bacteriol
- Publisher
- Wiley
- Copyright
- Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 1744-3091
- eISSN
- 1744-3091
- DOI
- 10.1107/S1744309106011456
- pmid
- 16682769
- Publisher site
- See Article on Publisher Site
Abstract
The Bacillus subtilis YphC gene encodes an essential GTPase thought to be involved in ribosome binding and whose protein product may represent a target for the development of a novel antibacterial agent. Sequence analysis reveals that YphC belongs to the EngA family of GTPases, which uniquely contain two adjacent GTP‐binding domains. Crystals of a selenomethionine‐incorporated YphC–GDP complex have been grown using the hanging‐drop vapour‐diffusion method and polyethylene glycol as a precipitating agent. The crystals belong to space group P212121, with unit‐cell parameters a = 62.71, b = 65.05, c = 110.61 Å, and have one molecule in the asymmetric unit. Data sets at three different wavelengths were collected on a single crystal to 2.5 Å resolution at the Daresbury SRS in order to solve the structure by MAD. Ultimately, analysis of YphC in complex with GDP may allow a greater understanding of the EngA family of essential GTPases.
Journal
Acta Crystallographica Section F – Wiley
Published: May 1, 2006