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Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis

Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of... Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 Å resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P61 or P65, with unit‐cell parameters a = b = 125, c = 54 Å. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section F Wiley

Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of macrophage growth locus A (MglA) protein from Francisella tularensis

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References (10)

Publisher
Wiley
Copyright
International Union of Crystallography, 2010
ISSN
1744-3091
eISSN
1744-3091
DOI
10.1107/S1744309110009711
pmid
20445258
Publisher site
See Article on Publisher Site

Abstract

Francisella tularensis, a potential bioweapon, causes a rare infectious disease called tularemia in humans and animals. The macrophage growth locus A (MglA) protein from F. tularensis associates with RNA polymerase to positively regulate the expression of multiple virulence factors that are required for its survival and replication within macrophages. The MglA protein was overproduced in Escherichia coli, purified and crystallized. The crystals diffracted to 7.5 Å resolution at the Advanced Photon Source, Argonne National Laboratory and belonged to the hexagonal space group P61 or P65, with unit‐cell parameters a = b = 125, c = 54 Å.

Journal

Acta Crystallographica Section FWiley

Published: May 1, 2010

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