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Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderrna pigmentosum

Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderrna... BY CLAUS R. BARTRAM, THEA KOSKE-WESTPHAL AND EBERHARD PASSARGE* Institut f u r Humangenetik, Universitat Hamburg Recent techniques to detect sister chromatid exchanges (SCE) in somatic metaphase chromosomes (Latt, 1973; Kato, 1974; Perry & Wolff, 1974; Wolff & Perry, 1974; Dutrillaux et al. 1974; Perry & Evans, 1975) afford an opportunity to examine chromosomal instability occurring in cultured cells of some hereditary disorders. The results of chromatid exchanges can be observed as differences in the uptake of certain stains by the two chromatids of each metaphase chromosome after the cell has replicated twice in the presence of 5-bromodeoxyuridine (BrdU) prior to harvest. This thymidine analogue, when incorporated into DNA, reduces the ultraviolet light fluorescence of fluorochromes such as Hoechst 33258 (Latt, 1973) or acridine orange (Kato, 1974; Perry & Wolff, 1974) bound to poly(da-dT) to about 20% when bound to poly(dA-BrdU). In addition, permanently Giemsa-stained preparations can be obtained following exposure to fluorescent dyes (Perry & Wolff, 1974; Wolff & Perry, 1974). Two cell cycles in the dark (to prevent artificial induction of SCEs by photolysis of BrdU-containing DNA) are required to observe a sharp demarcation between one brightly fluorescent or deeply Giemsa-stained chromatid, and one that is http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Human Genetics Wiley

Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderrna pigmentosum

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References (39)

Publisher
Wiley
Copyright
Copyright © 1976 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0003-4800
eISSN
1469-1809
DOI
10.1111/j.1469-1809.1976.tb00166.x
Publisher site
See Article on Publisher Site

Abstract

BY CLAUS R. BARTRAM, THEA KOSKE-WESTPHAL AND EBERHARD PASSARGE* Institut f u r Humangenetik, Universitat Hamburg Recent techniques to detect sister chromatid exchanges (SCE) in somatic metaphase chromosomes (Latt, 1973; Kato, 1974; Perry & Wolff, 1974; Wolff & Perry, 1974; Dutrillaux et al. 1974; Perry & Evans, 1975) afford an opportunity to examine chromosomal instability occurring in cultured cells of some hereditary disorders. The results of chromatid exchanges can be observed as differences in the uptake of certain stains by the two chromatids of each metaphase chromosome after the cell has replicated twice in the presence of 5-bromodeoxyuridine (BrdU) prior to harvest. This thymidine analogue, when incorporated into DNA, reduces the ultraviolet light fluorescence of fluorochromes such as Hoechst 33258 (Latt, 1973) or acridine orange (Kato, 1974; Perry & Wolff, 1974) bound to poly(da-dT) to about 20% when bound to poly(dA-BrdU). In addition, permanently Giemsa-stained preparations can be obtained following exposure to fluorescent dyes (Perry & Wolff, 1974; Wolff & Perry, 1974). Two cell cycles in the dark (to prevent artificial induction of SCEs by photolysis of BrdU-containing DNA) are required to observe a sharp demarcation between one brightly fluorescent or deeply Giemsa-stained chromatid, and one that is

Journal

Annals of Human GeneticsWiley

Published: Jul 1, 1976

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