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Chemical and lectin‐gold electron microscopical studies of the expression of the Galα1‐determinant in the pig aorta

Chemical and lectin‐gold electron microscopical studies of the expression of the... Abstract: In the xenotransplantation research field, pig aortic endothelial cells are frequently used in different model systems, e.g., for the study of the xenoantibody‐antigen reaction. The Galα1,3Gal determinant is the major target for human xenoreactive antibodies in pig tissue. Characterisation of the Galαl — distribution in pig aortic endothelial cells is thus important for understanding the reaction occurring at the endothelial cells during the xenorejection. We have determined the complete structure of the major Galα1,3Gal terminated glycolipid, Galα1,3nLc4Cer. Structural studies were performed on isolated glycosphingolipids by mass spectrometry and NMR spectroscopy. The results show a predominance of the pentasaccharide among the Galα1‐terminated glycolipids but also the presence of several Galα1‐terminated glycolipids with extended carbohydrate core chains. Ultrastructural localisation of the Galαl‐antigen in pig aorta was done by lectin‐gold electron microscopic studies of aortic wall sections using the Griffonia simplicifolia isolectin B4. Galα1‐determinants are predominantly localised on the luminal surface of pig aortic endothelial cells and endothelial cells of vasa vasorum and, to a lesser extent, vascular subendothelium. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Chemical and lectin‐gold electron microscopical studies of the expression of the Galα1‐determinant in the pig aorta

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References (44)

Publisher
Wiley
Copyright
Copyright © 1998 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.1998.tb00035.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: In the xenotransplantation research field, pig aortic endothelial cells are frequently used in different model systems, e.g., for the study of the xenoantibody‐antigen reaction. The Galα1,3Gal determinant is the major target for human xenoreactive antibodies in pig tissue. Characterisation of the Galαl — distribution in pig aortic endothelial cells is thus important for understanding the reaction occurring at the endothelial cells during the xenorejection. We have determined the complete structure of the major Galα1,3Gal terminated glycolipid, Galα1,3nLc4Cer. Structural studies were performed on isolated glycosphingolipids by mass spectrometry and NMR spectroscopy. The results show a predominance of the pentasaccharide among the Galα1‐terminated glycolipids but also the presence of several Galα1‐terminated glycolipids with extended carbohydrate core chains. Ultrastructural localisation of the Galαl‐antigen in pig aorta was done by lectin‐gold electron microscopic studies of aortic wall sections using the Griffonia simplicifolia isolectin B4. Galα1‐determinants are predominantly localised on the luminal surface of pig aortic endothelial cells and endothelial cells of vasa vasorum and, to a lesser extent, vascular subendothelium.

Journal

Xenotransplantation – Wiley

Published: Nov 1, 1998

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