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Cellulose accumulation mediated by PbrCSLD5, a cellulose synthase‐like protein, results in cessation of pollen tube growth in Pyrus bretschneideri

Cellulose accumulation mediated by PbrCSLD5, a cellulose synthase‐like protein, results in... Cellulose, a key component of the cell wall, plays an important role in maintaining the growth of pollen tubes. However, the molecular mechanism of cellulose participating in the cessation of pear pollen tube growth remains unclear. Here, we reported that at 15 h post‐cultured (HPC), the slow‐growth pear pollen tubes showed thickened cell walls and cellulose accumulation in the inner wall. Transcriptome data and quantitative real‐time PCR analysis showed that PbrCSLD5, a cellulose synthesis‐like gene, was highly expressed in the 15 HPC pear pollen tubes. Knockdown of PbrCSLD5 caused a decrease in cellulose content in pear pollen tubes. Moreover, PbrCSLD5 overexpression in Arabidopsis resulted in the accumulation of cellulose and disruption of normal pollen tube growth. Transcription factor PbrMADS52 was found to bind to the promoter of PbrCSLD5 and enhanced its expression. Our results suggested that the PbrMADS52–PbrCSLD5 signaling pathway led to increased cellulose content in the pear pollen tube cell wall, thereby inhibiting pollen tube growth. These results provided new insights into the regulation of pollen tube growth. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Physiologia Plantarum Wiley

Cellulose accumulation mediated by PbrCSLD5, a cellulose synthase‐like protein, results in cessation of pollen tube growth in Pyrus bretschneideri

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References (55)

Publisher
Wiley
Copyright
© 2022 Scandinavian Plant Physiology Society
ISSN
0031-9317
eISSN
1399-3054
DOI
10.1111/ppl.13700
Publisher site
See Article on Publisher Site

Abstract

Cellulose, a key component of the cell wall, plays an important role in maintaining the growth of pollen tubes. However, the molecular mechanism of cellulose participating in the cessation of pear pollen tube growth remains unclear. Here, we reported that at 15 h post‐cultured (HPC), the slow‐growth pear pollen tubes showed thickened cell walls and cellulose accumulation in the inner wall. Transcriptome data and quantitative real‐time PCR analysis showed that PbrCSLD5, a cellulose synthesis‐like gene, was highly expressed in the 15 HPC pear pollen tubes. Knockdown of PbrCSLD5 caused a decrease in cellulose content in pear pollen tubes. Moreover, PbrCSLD5 overexpression in Arabidopsis resulted in the accumulation of cellulose and disruption of normal pollen tube growth. Transcription factor PbrMADS52 was found to bind to the promoter of PbrCSLD5 and enhanced its expression. Our results suggested that the PbrMADS52–PbrCSLD5 signaling pathway led to increased cellulose content in the pear pollen tube cell wall, thereby inhibiting pollen tube growth. These results provided new insights into the regulation of pollen tube growth.

Journal

Physiologia PlantarumWiley

Published: May 1, 2022

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