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Autoradiographic localization of proline uptake in excitatory hippocampal pathways

Autoradiographic localization of proline uptake in excitatory hippocampal pathways An autoradiographic method was developed to localize sites of high‐affinity, Na+‐dependent proline uptake in the rat hippocampal formation. Hippocampal slices were incubated with [3H]proline, fixed with a glutaraldehyde/carbodiimide mixture, and cut into frozen sections. The sections were coated with photographic emulsion and autoradiograms were prepared. Autoradiographic grain densities were highest over the inner and outer thirds of the dentate molecular layer, followed by stratum lacunosum‐moleculare of area CA3. Stratum oriens and stratum radiatum of area CA1 and CA3 were fairly intensely labeled. The pyramidal and granule cell body layers, stratum lucidum of area CA3, and middle third of the dentate molecular layer were lightly labeled. Effects of surgical and kainic acid lesions suggested that the lateral perforant path, associational‐commissural fibers in the fascia dentata, and Schaffer collateral‐commissural‐ipsilateral stratum oriens fibers have considerable proline uptake capacity. In contrast, the medial perforant path and the mossy fibers appear to accumulate little or no proline. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Hippocampus Wiley

Autoradiographic localization of proline uptake in excitatory hippocampal pathways

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References (62)

Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services
ISSN
1050-9631
eISSN
1098-1063
DOI
10.1002/hipo.450020306
pmid
1308188
Publisher site
See Article on Publisher Site

Abstract

An autoradiographic method was developed to localize sites of high‐affinity, Na+‐dependent proline uptake in the rat hippocampal formation. Hippocampal slices were incubated with [3H]proline, fixed with a glutaraldehyde/carbodiimide mixture, and cut into frozen sections. The sections were coated with photographic emulsion and autoradiograms were prepared. Autoradiographic grain densities were highest over the inner and outer thirds of the dentate molecular layer, followed by stratum lacunosum‐moleculare of area CA3. Stratum oriens and stratum radiatum of area CA1 and CA3 were fairly intensely labeled. The pyramidal and granule cell body layers, stratum lucidum of area CA3, and middle third of the dentate molecular layer were lightly labeled. Effects of surgical and kainic acid lesions suggested that the lateral perforant path, associational‐commissural fibers in the fascia dentata, and Schaffer collateral‐commissural‐ipsilateral stratum oriens fibers have considerable proline uptake capacity. In contrast, the medial perforant path and the mossy fibers appear to accumulate little or no proline.

Journal

HippocampusWiley

Published: Jan 1, 1992

Keywords: ; ; ; ;

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