Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Auswahl der Inkubationszeit bei Bestimmung der Belebtschlammaktivität mit Hilfe des TTC‐Tests

Auswahl der Inkubationszeit bei Bestimmung der Belebtschlammaktivität mit Hilfe des TTC‐Tests The general assumption that the quantity of the formazan formed is linearly proportional to the time of incubation cannot be confirmed in practice. A formation of formazan which is too low initially is due to the initially passive transport of TTC to the sites of action. A formazan formation which is initially very high occurs through non‐enzymatic reduction of TTC by reducing substances in the environment outside the bacterial cells. During longer periods of incubation, especially during toxicological tests, the substrate may get exhausted, so that less formazan will be formed. For the correct determination of the dehydrogenase activity by means of TTC it is necessary to determine the period of incubation within which the increase of formazan is linearly proportional to time. The dehydrogenase activity can be exactly determined only from this period of time of incubation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta hydrochimica et hydrobiologica Wiley

Auswahl der Inkubationszeit bei Bestimmung der Belebtschlammaktivität mit Hilfe des TTC‐Tests

Miksch, K.
Acta hydrochimica et hydrobiologica , Volume 16 (6) – Jan 1, 1988
Download PDF
9 pages

Loading next page...
 
/lp/wiley/auswahl-der-inkubationszeit-bei-bestimmung-der-belebtschlammaktivit-t-kwc1btJPe6

References (2)

Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0323-4320
eISSN
1521-401X
DOI
10.1002/aheh.19880160609
Publisher site
See Article on Publisher Site

Abstract

The general assumption that the quantity of the formazan formed is linearly proportional to the time of incubation cannot be confirmed in practice. A formation of formazan which is too low initially is due to the initially passive transport of TTC to the sites of action. A formazan formation which is initially very high occurs through non‐enzymatic reduction of TTC by reducing substances in the environment outside the bacterial cells. During longer periods of incubation, especially during toxicological tests, the substrate may get exhausted, so that less formazan will be formed. For the correct determination of the dehydrogenase activity by means of TTC it is necessary to determine the period of incubation within which the increase of formazan is linearly proportional to time. The dehydrogenase activity can be exactly determined only from this period of time of incubation.

Journal

Acta hydrochimica et hydrobiologica – Wiley

Published: Jan 1, 1988

There are no references for this article.