Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Analysis of human CD4 T lymphocyte proliferation induced by porcine lymphoblastoid B cell lines

Analysis of human CD4 T lymphocyte proliferation induced by porcine lymphoblastoid B cell lines Bonenfant C, Vallée I, Sun J, Brossay A, Thibault G, Guillaumin JM, Lebranchu Y, Bardos P, Butler JE, Watier H. Analysis of human CD4 T lymphocyte proliferation induced by porcine lymphoblastoid B cell lines. Xenotransplantation 2003; 10: 107–119. © Blackwell Munksgaard, 2003 Background: This study was undertaken to characterize the two porcine lymphoblastoid cell lines L23 and L35, derived from a pig inoculated by the retrovirus Tsukuba‐1, and to determine how they induce a strong human lymphocyte proliferation. Methods: Phenotypic characterization was performed by flow cytometry and reverse transcriptase‐polymerase chain reaction analyses. Xenogeneic mixed lymphocyte reactions (XMLR) were performed using unfractionated human peripheral blood mononuclear cells (huPBMC) and purified CD4+ T lymphocytes as responding cells, in the presence of blocking antibodies and fusion proteins. Results: The immunoglobulin genes were demonstrated to be rearranged in L23 and L35 cell lines, in agreement with the expression of a B cell phenotype. Both induced a similar proliferation of huPBMCs and purified human CD4+ lymphocytes from adult or cord blood (naïve cells). Proliferation of CD4+ T lymphocytes was completely blocked by anti‐SLA‐DR plus anti‐SLA‐DQ mAbs, excluding human lymphocyte transformation by porcine viruses. The frequency of proliferative precursors was inconsistent with that induced by a retroviral superantigen but similar to classical direct xenoantigen presentation as observed with other porcine antigen‐presenting cells. Extensive analysis of costimulatory signals led to the identification of the CD28 pathway, in agreement with membrane expression of B7 molecules on L23 and L35 cells, and of the CD2 pathway in L35 cells. Conclusion: These two porcine lymphoblastoid cell lines have been further characterized and clearly identified as belonging to the B cell lineage. By expressing major histocompatibility complex class II antigens and costimulatory molecules, they induce a vigorous proliferative response of human CD4+ lymphocytes through a direct presentation pathway. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

Analysis of human CD4 T lymphocyte proliferation induced by porcine lymphoblastoid B cell lines

Download PDF
13 pages

Loading next page...
 
/lp/wiley/analysis-of-human-cd4-t-lymphocyte-proliferation-induced-by-porcine-eBngejZ9lv

References (72)

Publisher
Wiley
Copyright
Copyright © 2003 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1034/j.1399-3089.2003.01062.x
Publisher site
See Article on Publisher Site

Abstract

Bonenfant C, Vallée I, Sun J, Brossay A, Thibault G, Guillaumin JM, Lebranchu Y, Bardos P, Butler JE, Watier H. Analysis of human CD4 T lymphocyte proliferation induced by porcine lymphoblastoid B cell lines. Xenotransplantation 2003; 10: 107–119. © Blackwell Munksgaard, 2003 Background: This study was undertaken to characterize the two porcine lymphoblastoid cell lines L23 and L35, derived from a pig inoculated by the retrovirus Tsukuba‐1, and to determine how they induce a strong human lymphocyte proliferation. Methods: Phenotypic characterization was performed by flow cytometry and reverse transcriptase‐polymerase chain reaction analyses. Xenogeneic mixed lymphocyte reactions (XMLR) were performed using unfractionated human peripheral blood mononuclear cells (huPBMC) and purified CD4+ T lymphocytes as responding cells, in the presence of blocking antibodies and fusion proteins. Results: The immunoglobulin genes were demonstrated to be rearranged in L23 and L35 cell lines, in agreement with the expression of a B cell phenotype. Both induced a similar proliferation of huPBMCs and purified human CD4+ lymphocytes from adult or cord blood (naïve cells). Proliferation of CD4+ T lymphocytes was completely blocked by anti‐SLA‐DR plus anti‐SLA‐DQ mAbs, excluding human lymphocyte transformation by porcine viruses. The frequency of proliferative precursors was inconsistent with that induced by a retroviral superantigen but similar to classical direct xenoantigen presentation as observed with other porcine antigen‐presenting cells. Extensive analysis of costimulatory signals led to the identification of the CD28 pathway, in agreement with membrane expression of B7 molecules on L23 and L35 cells, and of the CD2 pathway in L35 cells. Conclusion: These two porcine lymphoblastoid cell lines have been further characterized and clearly identified as belonging to the B cell lineage. By expressing major histocompatibility complex class II antigens and costimulatory molecules, they induce a vigorous proliferative response of human CD4+ lymphocytes through a direct presentation pathway.

Journal

Xenotransplantation – Wiley

Published: Mar 1, 2003

There are no references for this article.