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Additive effects of CD59 expression in Gal knockout mice in vitro but not in an ex vivo model

Additive effects of CD59 expression in Gal knockout mice in vitro but not in an ex vivo model Abstract: Transgenic expression of the human complement regulatory molecule CD59 in mice and genetic deletion of the major xenoantigen galactose α 1,3 galactose (Gal KO) each resulted in partial protection of spleen cells from lysis by human serum. These protective effects were additive when the two genetic modifications were combined. However, when the effects of these genetic modifications were examined in an ex vivo model in which mouse hearts were perfused with human plasma, it was Gal KO which was the modification which determined protection. CD59 expression alone was not protective and CD59 expression in combination with Gal knockout did not result in a significant additional increase in protection over and above that provided by Gal knockout alone. The likely explanation for this discrepancy between the in vitro and ex vivo data is that the H2‐Kb promoter used to drive CD59 expression results I in substantially less expression on endothelium than on spleen cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Xenotransplantation Wiley

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References (29)

Publisher
Wiley
Copyright
© 1997 Munksgaard
ISSN
0908-665X
eISSN
1399-3089
DOI
10.1111/j.1399-3089.1997.tb00161.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Transgenic expression of the human complement regulatory molecule CD59 in mice and genetic deletion of the major xenoantigen galactose α 1,3 galactose (Gal KO) each resulted in partial protection of spleen cells from lysis by human serum. These protective effects were additive when the two genetic modifications were combined. However, when the effects of these genetic modifications were examined in an ex vivo model in which mouse hearts were perfused with human plasma, it was Gal KO which was the modification which determined protection. CD59 expression alone was not protective and CD59 expression in combination with Gal knockout did not result in a significant additional increase in protection over and above that provided by Gal knockout alone. The likely explanation for this discrepancy between the in vitro and ex vivo data is that the H2‐Kb promoter used to drive CD59 expression results I in substantially less expression on endothelium than on spleen cells.

Journal

XenotransplantationWiley

Published: Feb 1, 1997

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