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Abstracts

Abstracts 7 Protein expression analysis on human embryonic carcinoma (NCCIT) cell‐derived neuronal cells line with treatment of fluoxetine DY Oh, 1 JH Choi, 2,3 YC Park 2,3 1 Department of Psychiatry, CHA Bundang Medical Center, CHA University, Bundang‐gu, Seongnam‐si, Gyeonggi‐do, South Korea 2 Department of Neuropsychiatry, College of Medicine, Hanyang University and Hanyang University Guri Hospital, Seoul, South Korea 3 Department of Neuropsychiatry and Mental Health Institute, College of Medicine, Hanyang University, Seoul, South Korea Introduction: Embryonic stem (ES) cells in their gene expression profile as well as self‐renewal capacity and the potential to differentiate into three embryonic germ layers. 1 Adult hippocampal neurogenesis hypothesis states that the generation of neurons in the postnatal brain is important for understanding and treating anxiety disorders, like post‐traumatic stress disorder (PTSD). 2 The aim of this study was to identify the protein expression characteristics in NCCIT cell‐derived neurons after long‐term treatment (7 days) with fluoxetine, which is well‐known for treatment of anxiety disorders. Materials and methods: EBs from NCCIT cells (CRL‐2073) were incubated with 10 μM retinoic acid and 10 μM fluoxetine for 7 days. Proteins samples were subjected to two‐dimensional gel electrophoresis (2‐DE) using PDQuest software to quantify the spot densities. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Asia-Pacific Psychiatry Wiley

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Publisher
Wiley
Copyright
© 2015 Wiley Publishing Asia Pty Ltd
ISSN
1758-5864
eISSN
1758-5872
DOI
10.1111/appy.12194
pmid
26059281
Publisher site
See Article on Publisher Site

Abstract

7 Protein expression analysis on human embryonic carcinoma (NCCIT) cell‐derived neuronal cells line with treatment of fluoxetine DY Oh, 1 JH Choi, 2,3 YC Park 2,3 1 Department of Psychiatry, CHA Bundang Medical Center, CHA University, Bundang‐gu, Seongnam‐si, Gyeonggi‐do, South Korea 2 Department of Neuropsychiatry, College of Medicine, Hanyang University and Hanyang University Guri Hospital, Seoul, South Korea 3 Department of Neuropsychiatry and Mental Health Institute, College of Medicine, Hanyang University, Seoul, South Korea Introduction: Embryonic stem (ES) cells in their gene expression profile as well as self‐renewal capacity and the potential to differentiate into three embryonic germ layers. 1 Adult hippocampal neurogenesis hypothesis states that the generation of neurons in the postnatal brain is important for understanding and treating anxiety disorders, like post‐traumatic stress disorder (PTSD). 2 The aim of this study was to identify the protein expression characteristics in NCCIT cell‐derived neurons after long‐term treatment (7 days) with fluoxetine, which is well‐known for treatment of anxiety disorders. Materials and methods: EBs from NCCIT cells (CRL‐2073) were incubated with 10 μM retinoic acid and 10 μM fluoxetine for 7 days. Proteins samples were subjected to two‐dimensional gel electrophoresis (2‐DE) using PDQuest software to quantify the spot densities.

Journal

Asia-Pacific PsychiatryWiley

Published: Jun 1, 2015

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