Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

A dual‐modal red‐emitting fluorescence probe for proteins based on modulation of AIE or TICT state

A dual‐modal red‐emitting fluorescence probe for proteins based on modulation of AIE or TICT state A cyanine fluorophore 2‐[4‐N,N‐diphenylaminostyryl]‐β‐naphthothiazolium propylsulfonate, N3, was synthesized. The asymmetric probe N3 displayed aggregation‐induced emission (AIE) characteristic in DMSO/H2O mixtures, whereas it presented twisted intra‐molecular charge transfer (TICT) non‐emissive state in Dioxane/H2O (Diox/H2O) mixtures. Both the AIE and TICT state could be modulated by proteins, exhibiting switch effect. In DMSO/H2O mixtures with 90% water fractions (fw), protein molecules adsorbed upon the aggregated crystalline nanoparticles of N3 and spread over the surface, thus the strong fluorescence emission of N3 was quenched. The different degree of quenching among proteins revealed that the adsorption of proteins on N3 aggregate particles arose from both hydrophobic and electrostatic interactions. The quenched ratio (I0/I) vs the concentration of positively protein lysozyme (Lys) gave a Langmuir type curve. In Diox/H2O mixtures with 90% fw, the addition of bovine serum albumin (BSA) led to the reduction of non‐emissive TICT state and made the fluorescence emission of N3 switch on. The large difference of N3 fluorescence emission toward BSA over other proteins including human serum albumin allowed us to establish a sensitive probe for BSA. An ON‐OFF and OFF‐ON dual‐modal red‐emitting fluorescence probe for proteins was established. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Heteroatom Chemistry Wiley

A dual‐modal red‐emitting fluorescence probe for proteins based on modulation of AIE or TICT state

Loading next page...
 
/lp/wiley/a-dual-modal-red-emitting-fluorescence-probe-for-proteins-based-on-O1aVkPeX04

References (38)

Publisher
Wiley
Copyright
Copyright © 2017 Wiley Periodicals, Inc.
ISSN
1042-7163
eISSN
1098-1071
DOI
10.1002/hc.21371
Publisher site
See Article on Publisher Site

Abstract

A cyanine fluorophore 2‐[4‐N,N‐diphenylaminostyryl]‐β‐naphthothiazolium propylsulfonate, N3, was synthesized. The asymmetric probe N3 displayed aggregation‐induced emission (AIE) characteristic in DMSO/H2O mixtures, whereas it presented twisted intra‐molecular charge transfer (TICT) non‐emissive state in Dioxane/H2O (Diox/H2O) mixtures. Both the AIE and TICT state could be modulated by proteins, exhibiting switch effect. In DMSO/H2O mixtures with 90% water fractions (fw), protein molecules adsorbed upon the aggregated crystalline nanoparticles of N3 and spread over the surface, thus the strong fluorescence emission of N3 was quenched. The different degree of quenching among proteins revealed that the adsorption of proteins on N3 aggregate particles arose from both hydrophobic and electrostatic interactions. The quenched ratio (I0/I) vs the concentration of positively protein lysozyme (Lys) gave a Langmuir type curve. In Diox/H2O mixtures with 90% fw, the addition of bovine serum albumin (BSA) led to the reduction of non‐emissive TICT state and made the fluorescence emission of N3 switch on. The large difference of N3 fluorescence emission toward BSA over other proteins including human serum albumin allowed us to establish a sensitive probe for BSA. An ON‐OFF and OFF‐ON dual‐modal red‐emitting fluorescence probe for proteins was established.

Journal

Heteroatom ChemistryWiley

Published: May 1, 2017

Keywords: ; ; ; ;

There are no references for this article.