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A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of GFR with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine and plasma urea based equation to estimate GFR in a large cohort of male rats (development cohort n=325, R2=0.816, percentage of predictions that fall within 30% of the true value (p30)=76%) which had high accuracy in the validation cohort (n=116 rats, R2=0.935, p30=79%). The equation was less accurate in rats with non-steady-state creatinine in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Physiology-Renal Physiology The American Physiological Society

A plasma creatinine- and urea-based equation to estimate glomerular filtration rate in rats

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ISSN
1931-857x
eISSN
1522-1466
DOI
10.1152/ajprenal.00656.2020
Publisher site
See Article on Publisher Site

Abstract

Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of GFR with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine and plasma urea based equation to estimate GFR in a large cohort of male rats (development cohort n=325, R2=0.816, percentage of predictions that fall within 30% of the true value (p30)=76%) which had high accuracy in the validation cohort (n=116 rats, R2=0.935, p30=79%). The equation was less accurate in rats with non-steady-state creatinine in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats.

Journal

American Journal of Physiology-Renal Physiology – The American Physiological Society

Published: Mar 1, 2021

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