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C. Schou, N. Heegaard (2003)
Recent applications of affinity interactions in capillary electrophoresisElectrophoresis, 27
N. Chiem, D. Harrison (1997)
Microchip-based capillary electrophoresis for immunoassays: analysis of monoclonal antibodies and theophylline.Analytical chemistry, 69 3
E. Mothershed, A. Whitney (2006)
Nucleic acid-based methods for the detection of bacterial pathogens: present and future considerations for the clinical laboratory.Clinica chimica acta; international journal of clinical chemistry, 363 1-2
N. Chiem, D. Harrison (1998)
Monoclonal antibody binding affinity determined by microchip‐based capillary electrophoresisELECTROPHORESIS, 19
F. Paillard, C. Hill (2004)
Direct nucleic acid diagnostic tests for bacterial infectious diseases: streptococcal pharyngitis, pulmonary tuberculosis, vaginitis, chlamydial and gonococcal infections.MLO: medical laboratory observer, 36 1
D. Huber, M. Markel, S. Pennathur, K. Patel (2009)
Oligonucleotide hybridization and free-solution electrokinetic separation in a nanofluidic device.Lab on a chip, 9 20
Kelly Karns, A. Herr (2011)
Human tear protein analysis enabled by an alkaline microfluidic homogeneous immunoassay.Analytical chemistry, 83 21
K. Shimura, B. Karger (1994)
Affinity probe capillary electrophoresis: analysis of recombinant human growth hormone with a fluorescent labeled antibody fragment.Analytical chemistry, 66 1
MS Bhakta, DJ Segal (2010)
The generation of zinc finger proteins by modular assemblyEngineered zinc finger proteins
Yuchen Pan, Kelly Karns, A. Herr (2014)
Microfluidic electrophoretic mobility shift assays for quantitative biochemical analysisELECTROPHORESIS, 35
C. Hou, A. Herr (2010)
Ultrashort separation length homogeneous electrophoretic immunoassays using on-chip discontinuous polyacrylamide gels.Analytical chemistry, 82 8
N. Schultz, R. Kennedy (1993)
Rapid Immunoassays Using Capillary Electrophoresis with Fluorescence DetectionAnalytical Chemistry, 65
Siew Cheng, C. Skinner, Justine Taylor, S. Attiya, William Lee, G. Picelli, D. Harrison (2001)
Development of a multichannel microfluidic analysis system employing affinity capillary electrophoresis for immunoassay.Analytical chemistry, 73 7
D. Geisthardt, J. Kruppa (1987)
Polyacrylamide gel electrophoresis: reaction of acrylamide at alkaline pH with buffer components and proteins.Analytical biochemistry, 160 1
D. Segal, R. Beerli, P. Blancafort, B. Dreier, K. Effertz, A. Huber, B. Koksch, Caren Lund, L. Magnenat, David Valente, C. Barbas (2003)
Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins.Biochemistry, 42 7
Christiane König, Hans-Peter Simmen, Jürg Blaser (1998)
Bacterial concentrations in pus and infected peritoneal fluid--implications for bactericidal activity of antibiotics.The Journal of antimicrobial chemotherapy, 42 2
M. Schena, D. Shalon, Ronald Davis, P. Brown (1995)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayScience, 270
Y Pan (2014)
2078Electrophoresis, 35
Minsub Chung, Dohyun Kim, A. Herr (2014)
Polymer sieving matrices in microanalytical electrophoresis.The Analyst, 139 22
Bipasha Dey, Sameer Thukral, S. Krishnan, Mainak Chakrobarty, Sahil Gupta, Chanchal Manghani, V. Rani (2012)
DNA–protein interactions: methods for detection and analysisMolecular and Cellular Biochemistry, 365
A. Herr, D. Throckmorton, A. Davenport, A. Singh (2005)
On-chip native gel electrophoresis-based immunoassays for tetanus antibody and toxin.Analytical chemistry, 77 2
D. Schmalzing, W. Nashabeh (1997)
Capillary electrophoresis based immunoassays: A critical reviewELECTROPHORESIS, 18
L. Gorgannezhad, H. Stratton, N. Nguyen (2019)
Microfluidic-Based Nucleic Acid Amplification Systems in MicrobiologyMicromachines, 10
Dohyun Kim, Kelly Karns, S. Tia, M. He, A. Herr (2012)
Electrostatic protein immobilization using charged polyacrylamide gels and cationic detergent microfluidic Western blotting.Analytical chemistry, 84 5
B. Kuswandi, Nuriman, J. Huskens, W. Verboom (2007)
Optical sensing systems for microfluidic devices: a review.Analytica chimica acta, 601 2
G. Vahedi, K. Kaler, C. Backhouse (2004)
An integrated method for mutation detection using on‐chip sample preparation, single‐stranded conformation polymorphism, and heteroduplex analysisELECTROPHORESIS, 25
Seojin Bae, Kukhui Son, Doyoung Lee, Sangeun Han, Kyunghak Choi, Sanghyo Kim (2020)
Warfarin Pharmacogenetics: Single-nucleotide Polymorphism Detection using CMOS Photosensor-based Real-time PCRBioChip Journal, 14
Moon-Soo Kim, Gulnaz Stybayeva, J. Lee, A. Revzin, D. Segal (2010)
A zinc finger protein array for the visual detection of specific DNA sequences for diagnostic applicationsNucleic Acids Research, 39
Peng Liu, R. Mathies (2009)
Integrated microfluidic systems for high-performance genetic analysis.Trends in biotechnology, 27 10
(2016)
Isolation and identification of Methicillin and Vancomycin resistance Staphylococcus aureus from pus samples of injured skin patients in Lahore Pakistan
Xinya He, Yongsheng Ding, Dazhi Li, B. Lin (2004)
Recent advances in the study of biomolecular interactions by capillary electrophoresisELECTROPHORESIS, 25
E. Biet, Jian-Sheng Sun, M. Dutreix (1999)
Conserved sequence preference in DNA binding among recombination proteins: an effect of ssDNA secondary structure.Nucleic acids research, 27 2
A Bhatia (2007)
188J. Clin. Diagn. Res., 3
C. Hou, A. Herr (2008)
Clinically relevant advances in on‐chip affinity‐based electrophoresis and electrochromatographyELECTROPHORESIS, 29
I. Ghosh, Cliff Stains, A. Ooi, D. Segal (2006)
Direct detection of double-stranded DNA: Molecular methods and applications for DNA diagnostics.Molecular bioSystems, 2 11
Ying Lu, Shan Chen, Li Wei, Lanhua Sun, Hou-ming Liu, Youchun Xu (2019)
A Microfluidic-Based SNP Genotyping Method for Hereditary Hearing-Loss Detection.Analytical chemistry, 91 9
J. Xian, M. Harrington, E. Davidson (1996)
DNA-protein binding assays from a single sea urchin egg: a high-sensitivity capillary electrophoresis method.Proceedings of the National Academy of Sciences of the United States of America, 93 1
D. Neville (1971)
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.The Journal of biological chemistry, 246 20
R. Leimgruber, J. Malone, M. Radabaugh, M. Laporte, B. Violand, J. Monahan (2002)
Development of improved cell lysis, solubilization and imaging approaches for proteomic analysesPROTEOMICS, 2
A. Herr, Anson Hatch, D. Throckmorton, Huu Tran, J. Brennan, W. Giannobile, A. Singh (2007)
Microfluidic immunoassays as rapid saliva-based clinical diagnosticsProceedings of the National Academy of Sciences, 104
Hamin Na, Byoung-Hoon Kang, Jayoung Ku, Yoosik Kim, K. Jeong (2021)
On-chip Paper Electrophoresis for Ultrafast Screening of Infectious DiseasesBioChip Journal, 15
R. Meagher, Anson Hatch, R. Renzi, A. Singh (2008)
An integrated microfluidic platform for sensitive and rapid detection of biological toxins.Lab on a chip, 8 12
B. Dreier, R. Beerli, D. Segal, Jessica Flippin, Carlos Barbas (2001)
Development of Zinc Finger Domains for Recognition of the 5′-ANN-3′ Family of DNA Sequences and Their Use in the Construction of Artificial Transcription Factors*The Journal of Biological Chemistry, 276
R. Beerli, D. Segal, B. Dreier, Carlos Barbas (1998)
Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks.Proceedings of the National Academy of Sciences of the United States of America, 95 25
K. Gilligan, M. Shipley, B. Stiles, T. Hadfield, M. Ibrahim (2000)
Identification of Staphylococcus aureus enterotoxins A and B genes by PCR-ELISA.Molecular and cellular probes, 14 2
J. Bryan (1977)
Molecular weights of protein multimers from polyacrylamide gel electrophoresis.Analytical biochemistry, 78 2
M-S Kim (2010)
gkq1214Nucleic Acids Res., 39
Jiaming Hu, C. Easley (2011)
A simple and rapid approach for measurement of dissociation constants of DNA aptamers against proteins and small molecules via automated microchip electrophoresis.The Analyst, 136 17
Qiang Liu, David Segal, J. Ghiara, Carlos Barbas (1997)
Design of polydactyl zinc-finger proteins for unique addressing within complex genomes.Proceedings of the National Academy of Sciences of the United States of America, 94 11
Jin Kim, Minhee Kang, Eunkyoung Park, D. Chung, Jiyeon Kim, E. Hwang (2019)
A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoVBiochip Journal, 13
C. Ferreira, I. Pinto, E. Soares, H. Soares (2015)
(Un)suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions – a reviewRSC Advances, 5
A Bhatia, S Zahoor (2007)
Staphylococcus aureus enterotoxins: a reviewJ. Clin. Diagn. Res., 3
B. Bruijns, A. Asten, R. Tiggelaar, H. Gardeniers (2016)
Microfluidic Devices for Forensic DNA Analysis: A ReviewBiosensors, 6
David Segal, Birgit Dreier, R. Beerli, Carlos Barbas (1999)
Toward controlling gene expression at will: selection and design of zinc finger domains recognizing each of the 5'-GNN-3' DNA target sequences.Proceedings of the National Academy of Sciences of the United States of America, 96 6
Jarrod Clark, Taras Shevchuk, P. Swiderski, R. Dabur, L. Crocitto, Y. Buryanov, Steven Smith (2003)
Mobility-shift analysis with microfluidics chips.BioTechniques, 35 3
Hatice Koydemir, H. Külah, C. Özgen, A. Alp, G. Hasçelik (2011)
MEMS biosensors for detection of methicillin resistant Staphylococcus aureus.Biosensors & bioelectronics, 29 1
Katarzyna Kluska, Justyna Adamczyk, A. Krężel (2018)
Metal binding properties, stability and reactivity of zinc fingersCoordination Chemistry Reviews
D. Segal, C. Barbas (2001)
Custom DNA-binding proteins come of age: polydactyl zinc-finger proteins.Current opinion in biotechnology, 12 6
S. Brahmasandra, V. Ugaz, D. Burke, C. Mastrangelo, M. Burns (2001)
Electrophoresis in microfabricated devices using photopolymerized polyacrylamide gels and electrode‐defined sample injectionELECTROPHORESIS, 22
C. Hou, A. Herr (2013)
Microfluidic integration of Western blotting is enabled by electrotransfer-assisted sodium dodecyl sulfate dilution.The Analyst, 138 1
K. Lien, Gwo-Bin Lee (2010)
Miniaturization of molecular biological techniques for gene assay.The Analyst, 135 7
J. Hennekinne, M. Buyser, S. Dragacci (2012)
Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation.FEMS microbiology reviews, 36 4
F Paillard (2004)
10MLO Med. Lab. Obs., 36
M. He, A. Herr (2010)
Automated microfluidic protein immunoblottingNature Protocols, 5
M. Burns, Brian Johnson, S. Brahmasandra, K. Handique, J. Webster, M. Krishnan, T. Sammarco, Piu Man, Darren Jones, D. Heldsinger, C. Mastrangelo, D. Burke (1998)
An integrated nanoliter DNA analysis device.Science, 282 5388
A. Niemz, Tanya Ferguson, D. Boyle (2011)
Point-of-care nucleic acid testing for infectious diseases.Trends in biotechnology, 29 5
Dongjia Chen, Xiaoting Shen, Yan Xu, B. Cai, C. Ding, Yiping Zhong, Yanwen Xu, Canquan Zhou (2021)
Next-Generation Sequencing-Based Preimplantation Genetic Testing for De Novo NF1 MutationsBioChip Journal, 15
A. Klug, D. Rhodes (1987)
‘Zinc fingers’: a novel protein motif for nucleic acid recognitionTrends in Biochemical Sciences, 12
D. Bottenus, T. Jubery, Yexin Ouyang, W. Dong, P. Dutta, C. Ivory (2011)
10,000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis.Lab on a chip, 11 5
T. Duncombe, A. Herr (2013)
Photopatterned free-standing polyacrylamide gels for microfluidic protein electrophoresis.Lab on a chip, 13 11
S. Mangru, D. Harrison (1998)
Chemiluminescence detection in integrated post‐separation reactors for microchip‐based capillary electrophoresis and affinity electrophoresisELECTROPHORESIS, 19
G. Foulds, F. Etzkorn (1998)
A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution.Nucleic acids research, 26 18
A. Bromberg, R. Mathies (2004)
Multichannel homogeneous immunoassay for detection of 2,4,6‐trinitrotoluene (TNT) using a microfabricated capillary array electrophoresis chipELECTROPHORESIS, 25
Dat Ha, Sthitodhi Ghosh, C. Ahn, D. Segal, Moon-Soo Kim (2018)
Pathogen-specific DNA sensing with engineered zinc finger proteins immobilized on a polymer chip.The Analyst, 143 17
X. Brannmjk (2020)
CapillaryDefinitions
D. Schmalzing, L. Koutny, T. Taylor, W. Nashabeh, M. Fuchs (1997)
Immunoassay for thyroxine (T4) in serum using capillary electrophoresis and micromachined devices.Journal of chromatography. B, Biomedical sciences and applications, 697 1-2
Mital Bhakta, D. Segal (2010)
The generation of zinc finger proteins by modular assembly.Methods in molecular biology, 649
P. Smejkal, D. Bottenus, M. Breadmore, R. Guijt, C. Ivory, F. Foret, M. Macka (2013)
Microfluidic isotachophoresis: A reviewELECTROPHORESIS, 34
P. Hardenbol, J. Banér, Maneesh Jain, M. Nilsson, Eugeni Namsaraev, G. Karlin-Neumann, H. Fakhrai-Rad, M. Ronaghi, T. Willis, U. Landegren, Ronald Davis (2003)
Multiplexed genotyping with sequence-tagged molecular inversion probesNature Biotechnology, 21
Hao Yuan, Yingchao Liu, Xiran Jiang, Shangchen Xu, G. Sui (2014)
Microfluidic chip for rapid analysis of cerebrospinal fluid infected with Staphylococcus aureusAnalytical Methods, 6
L. Koutny, D. Schmalzing, T. Taylor, M. Fuchs (1996)
Microchip electrophoretic immunoassay for serum cortisol.Analytical chemistry, 68 1
We report for the first time a microfluidic electrophoretic mobility reversal assay (MEMRA) for double-stranded DNA (dsDNA) detection using zinc-finger proteins (ZFPs) and a polyacrylamide-gel (PAG) sieving matrix. Microfluidic DNA analysis was actively studied because of its importance in biology and medicine. Most microfluidic DNA detection techniques rely on time-consuming denaturation and hybridization processes. To address this limitation, ZFP was employed as a novel affinity probe, which directly binds to a specific sequence of dsDNA without denaturation and renaturation. A mildly alkaline electrophoresis buffer (pH 8.6) was used for our MEMRA, instead of a strongly alkaline buffer (pH 10.75) for separating the ZFP–dsDNA complex from interfering species. At pH 8.6, the mobility of ZFP was reversed upon binding with dsDNA (complex pI = ~ 5.33), and unbound ZFP (pI = ~ 9.3) was excluded from loading. Therefore, the ZFP–dsDNA complex was detected without zone interferences. Furthermore, nonspecific interactions and band dispersion, observed in strongly alkaline buffer, were effectively mitigated in the MEMRA. The ZFP–dsDNA complex was fully separated (separation resolution ≥ 2.0) and detected rapidly (12–15 s at a separation distance of 160–240 μm) using on-chip photopatterned 3–16%T discontinuous PAG. The MEMRA performance was excellent, providing a detection limit of 50 pM and a detection range of 100 pM–500 nM for seb (Staphylococcus enterotoxin B) gene dsDNA oligonucleotides. We expect that our ZFP-based MEMRA will find broad utility in biology and medicine where the rapid, specific, and quantitative detection of dsDNA is of paramount importance.
BioChip Journal – Springer Journals
Published: Dec 1, 2021
Keywords: Microchip electrophoresis; Electrophoretic homogeneous affinity assay; Double-stranded DNA analysis; Staphylococcus aureus; Zinc-finger protein
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