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Vascular wilt of basil in Australia

Vascular wilt of basil in Australia Fusarium wilt of basil, caused by Fusarium oxysporum f. sp. basilici, occurs worldwide but has not been officially recorded in Australia. Fourteen isolates of F. oxysporum were collected from diseased basil from a number of locations in Australia. A PCR diagnostic test previously shown to react only with isolates of F. oxysporum f. sp. basilici was used to identify the isolates and Koch’s postulates were performed for a subset of isolates to confirm pathogenicity. Two techniques, vegetative compatibility grouping and DNA fingerprinting using enterobacterial repetitive intergenic consensus (ERIC) targeted primers, were used to analyse all isolates. Diagnostic PCR confirmed that 11 of the isolates were F. oxysporum f. sp. basilici and they were shown to belong to a single VCG and to have a single ERIC profile, indicating that the population is essentially clonal. This is the first report of this pathogen in Australia. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australasian Plant Pathology Springer Journals

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References (19)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Australasian Plant Pathology Society
Subject
Life Sciences; Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology
ISSN
0815-3191
eISSN
1448-6032
DOI
10.1071/AP05100
Publisher site
See Article on Publisher Site

Abstract

Fusarium wilt of basil, caused by Fusarium oxysporum f. sp. basilici, occurs worldwide but has not been officially recorded in Australia. Fourteen isolates of F. oxysporum were collected from diseased basil from a number of locations in Australia. A PCR diagnostic test previously shown to react only with isolates of F. oxysporum f. sp. basilici was used to identify the isolates and Koch’s postulates were performed for a subset of isolates to confirm pathogenicity. Two techniques, vegetative compatibility grouping and DNA fingerprinting using enterobacterial repetitive intergenic consensus (ERIC) targeted primers, were used to analyse all isolates. Diagnostic PCR confirmed that 11 of the isolates were F. oxysporum f. sp. basilici and they were shown to belong to a single VCG and to have a single ERIC profile, indicating that the population is essentially clonal. This is the first report of this pathogen in Australia.

Journal

Australasian Plant PathologySpringer Journals

Published: Jan 28, 2011

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