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Validation of avenanthramide and other phenolic compounds in oats and sprouted oats and their antimicrobial properties against Escherichia coli O157:H7

Validation of avenanthramide and other phenolic compounds in oats and sprouted oats and their... Oat contains a variety of phenolic compounds, including avenanthramides, which are found only in oats. This study was conducted to establish the quantitative analysis of seven phenolic compounds in oat powders and sprouted oat powders and validate an efficient high-performance liquid chromatography (HPLC) method. All calibration curves represented good linearity (R2 ≥ 0.9997) in the concentration range (0.5−50 mg/kg) with LOD and LOQ of 0.01−0.21 and 0.02−0.64 mg/kg, respectively. Intra-day accuracy (%) and precision (%RSD) were 90.7−103.8% and 1.5−4.9%, respectively. Inter-day accuracy (%) and precision (%RSD) were 90.4−107.9% and 2.2−4.8%, respectively. Moreover, relative expanded measurement uncertainty results complied with CODEX guideline. In addition, selected oat and sprouted oat powder extracts showed anti-microbial activities against Escherichia coli O157:H7. These results demonstrated that this HPLC method is suitable for the qualification and quantification of seven phenolic compounds in oat and sprouted oat. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Science and Biotechnology Springer Journals

Validation of avenanthramide and other phenolic compounds in oats and sprouted oats and their antimicrobial properties against Escherichia coli O157:H7

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References (37)

Publisher
Springer Journals
Copyright
Copyright © The Korean Society of Food Science and Technology 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
1226-7708
eISSN
2092-6456
DOI
10.1007/s10068-022-01099-8
Publisher site
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Abstract

Oat contains a variety of phenolic compounds, including avenanthramides, which are found only in oats. This study was conducted to establish the quantitative analysis of seven phenolic compounds in oat powders and sprouted oat powders and validate an efficient high-performance liquid chromatography (HPLC) method. All calibration curves represented good linearity (R2 ≥ 0.9997) in the concentration range (0.5−50 mg/kg) with LOD and LOQ of 0.01−0.21 and 0.02−0.64 mg/kg, respectively. Intra-day accuracy (%) and precision (%RSD) were 90.7−103.8% and 1.5−4.9%, respectively. Inter-day accuracy (%) and precision (%RSD) were 90.4−107.9% and 2.2−4.8%, respectively. Moreover, relative expanded measurement uncertainty results complied with CODEX guideline. In addition, selected oat and sprouted oat powder extracts showed anti-microbial activities against Escherichia coli O157:H7. These results demonstrated that this HPLC method is suitable for the qualification and quantification of seven phenolic compounds in oat and sprouted oat.

Journal

Food Science and Biotechnology – Springer Journals

Published: Aug 1, 2022

Keywords: Avenanthramide; Phenolic compound; Oat; Sprouted oat; High-performance liquid chromatography; Measurement uncertainty; Quantification

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