Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

UV-B Radiation Response on Inducible Nitric Oxide Synthase and Antioxidant Defense System in Heteropneustes fossilis

UV-B Radiation Response on Inducible Nitric Oxide Synthase and Antioxidant Defense System in... Ultraviolet radiation is one of the baleful radiations of the Sun and among the classes of UV radiations, UV-B (280–315 nm) of wavelength is more destructive in aquatic ecosystems and considered to be a risk factor for organisms. In this study, we examined the level of oxidative stress in freshwater Singhi catfish, Heteropneustes fossilis exposed to UV-B radiation for different time intervals and for different experimental regime (in days) by measuring the level of Nitric Oxide enzyme, expression of inducible nitric oxide synthase (inos) gene and action of antioxidant defense mechanism in situ, in the tissues from liver, kidney, gills, muscle, intestine, brain, spleen and skin. We found elevated level of inos enzyme production owing to transcriptional induction of inos gene under experimental UV-radiation and subsequent accumulation of NO in the different tissues of Singhi catfish. The inos gene expression was found to be up-regulated in the liver, muscle, kidney, spleen, brain, and gills while it was downregulated in the skin. The result of the study correlates oxidative stress-induced up-regulation of the inos gene at transcriptional level with higher activity of inos in different tissues. There was a significant increase of GST, SOD, CAT along with lipid peroxidation and production of MDA in these tissues whereas, the level of GSH was found to be decreased. This study demonstrated that exposure to UV-B radiation affects the fishes but its robust antioxidant defense system it has enabled them to withstand this stress condition to a great extent. This study helps to design strategies in the culture of catfish to mitigate the ill effects of UV-B radiation exposure in near future. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the Zoological Society Springer Journals

UV-B Radiation Response on Inducible Nitric Oxide Synthase and Antioxidant Defense System in Heteropneustes fossilis

Loading next page...
 
/lp/springer-journals/uv-b-radiation-response-on-inducible-nitric-oxide-synthase-and-QtIp7hra77
Publisher
Springer Journals
Copyright
Copyright © Zoological Society, Kolkata, India 2021
ISSN
0373-5893
eISSN
0974-6919
DOI
10.1007/s12595-021-00372-8
Publisher site
See Article on Publisher Site

Abstract

Ultraviolet radiation is one of the baleful radiations of the Sun and among the classes of UV radiations, UV-B (280–315 nm) of wavelength is more destructive in aquatic ecosystems and considered to be a risk factor for organisms. In this study, we examined the level of oxidative stress in freshwater Singhi catfish, Heteropneustes fossilis exposed to UV-B radiation for different time intervals and for different experimental regime (in days) by measuring the level of Nitric Oxide enzyme, expression of inducible nitric oxide synthase (inos) gene and action of antioxidant defense mechanism in situ, in the tissues from liver, kidney, gills, muscle, intestine, brain, spleen and skin. We found elevated level of inos enzyme production owing to transcriptional induction of inos gene under experimental UV-radiation and subsequent accumulation of NO in the different tissues of Singhi catfish. The inos gene expression was found to be up-regulated in the liver, muscle, kidney, spleen, brain, and gills while it was downregulated in the skin. The result of the study correlates oxidative stress-induced up-regulation of the inos gene at transcriptional level with higher activity of inos in different tissues. There was a significant increase of GST, SOD, CAT along with lipid peroxidation and production of MDA in these tissues whereas, the level of GSH was found to be decreased. This study demonstrated that exposure to UV-B radiation affects the fishes but its robust antioxidant defense system it has enabled them to withstand this stress condition to a great extent. This study helps to design strategies in the culture of catfish to mitigate the ill effects of UV-B radiation exposure in near future.

Journal

Proceedings of the Zoological SocietySpringer Journals

Published: Jun 21, 2021

Keywords: inos gene; Nitric oxide synthase; Oxidative stress; Antioxidant defense mechanism

References