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Toxicity of Mercaptoethanol to Mutant Strains of the Yeast Pichia methanolica Growing on Different Carbon Sources

Toxicity of Mercaptoethanol to Mutant Strains of the Yeast Pichia methanolica Growing on... Addition of β-mercaptoethanol at a concentration of 2–3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeastPichia methanolica; mercaptoethanol at a concentration of 0.7 to 25 mM inhibited the growth of the mutant strain ecr1. The mutation mth1 of P. methanolica repressed its ability to consume methanol and was accompanied by the loss of alcohol oxidase (EC 1.1.3.13) activity. β-Mercaptoethanol restored the ability of mth1 mutant cells to grow on methanol and stimulated their growth under derepression conditions. The growth effect of β-mercaptoethanol during derepression was accompanied by partial restoration of alcohol oxidase activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Toxicity of Mercaptoethanol to Mutant Strains of the Yeast Pichia methanolica Growing on Different Carbon Sources

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References (9)

Publisher
Springer Journals
Copyright
Copyright © 2001 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Medical Microbiology; Biochemistry, general; Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1023/A:1002856828177
Publisher site
See Article on Publisher Site

Abstract

Addition of β-mercaptoethanol at a concentration of 2–3 mM to media containing methanol, glucose, or yeast extract caused a 50% inhibition of the growth of wild-type yeastPichia methanolica; mercaptoethanol at a concentration of 0.7 to 25 mM inhibited the growth of the mutant strain ecr1. The mutation mth1 of P. methanolica repressed its ability to consume methanol and was accompanied by the loss of alcohol oxidase (EC 1.1.3.13) activity. β-Mercaptoethanol restored the ability of mth1 mutant cells to grow on methanol and stimulated their growth under derepression conditions. The growth effect of β-mercaptoethanol during derepression was accompanied by partial restoration of alcohol oxidase activity.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Oct 19, 2004

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