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- Publisher
- Springer Journals
- Copyright
- Copyright © The Korean Fiber Society 2001
- ISSN
- 1229-9197
- eISSN
- 1875-0052
- DOI
- 10.1007/bf02875260
- Publisher site
- See Article on Publisher Site
Abstract
A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its biological stability and enhance its mechanical properties. The crosslinking medium had a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.
Journal
Fibers and Polymers – Springer Journals
Published: Jun 1, 2001
Keywords: Collagen; Chitosan; Scaffold; Sponge; Pore; Mechanical properties; CHO cell; Tissue engineering