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The review describes the classification, analysis, characteristics, and prospects of the application of various types of matrixes, of natural or synthetic origin, for cell biotechnology. Particular attention is paid to three-dimensional structures designed on the basis of extracellular matrix (ECM) components with multipotent mesenchymal stromal cells (MMSCs). In particular, the authors previously obtained cell populations with a phenotype similar to MMSCs from farm animal bone marrow (BM) and adipose tissues (AT). A comparative analysis of the properties and features of the derived cellular populations was performed. It was established that MMSCs isolated from BM and AT were capable of forming fatty, bone, and muscle tissue cells when cultured in induction media in vitro. Therefore, the production of farm animal muscle tissue analogs by myogenesis via three-dimensional MMSC cultivation on a porous matrix is a promising research direction for the needs of regenerative medicine, veterinary medicine, and cell and food biotechnology.
Applied Biochemistry and Microbiology – Springer Journals
Published: Oct 31, 2015
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