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Doklady Chemistry, Vol. 404, Part 1, 2005, pp. 178–181. Translated from Doklady Akademii Nauk, Vol. 404, No. 3, 2005, pp. 348–351. Original Russian Text Copyright © 2005 by Nesterenko, Rybalko. CHEMISTRY The Use of Ultrashort Monolithic Porous Columns and an Eluent Flow Gradient for Chromatographic Separation of Polyaromatic Hydrocarbons P. N. Nesterenko and M. A. Rybalko Presented by Academician Yu.A. Zolotov February 8, 2005 Received March 3, 2005 An important task of state-of-the-art high perfor- ensure a high load capacity [3]. This structure allows mance liquid chromatography (HPLC) is to decrease the one to reduce the time of chromatographic analysis time of analysis. There exist several approaches to solv- five- to sixfold with respect to the classical columns ing this task. The first one implies the use of short packed with microspherical particles. Indeed, a 10-cm- (3−5 cm long) columns packed with particles 1.5–3 µm long monolithic porous column 4.6 mm in diameter in diameter [1]. These tiny particles ensure an effective allows the separation of five β-blockers in 1 min at an mass transfer and rather high column efficiency. Com- eluent flow rate of 9 mL/min, the resolution of the col- plete separation of five inorganic anions requires
Doklady Chemistry – Springer Journals
Published: Oct 3, 2005
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