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The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile Flow and Cyclic Strain, In Vitro

The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile... The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile Flow and Cyclic Strain, In Vitro Olga I. Tsukurov, MD, Christopher J. Kwolek, MD, Gilbert J. L’Italien, PhD, Aziz Benbrahim, MD, Barbara B. Milinazzo, BS, Nancy E. Conroy, BS, Jonathan P. Gertler, MD, Roslyn W. Orkin, PhD, and William M. Abbott, MD, Boston, Massachusetts Adult human saphenous vein endothelial cells (HVEC) were cultured in a compliant tubular device and evaluated by Northern hybridization for the effects of combined pressurized pulsatile flow and cyclic strain on the expression of mRNAs for endothelin-1 (ET-1), endothelial cell nitric oxide synthase (ecNOS), tissue plasminogen activator (tPA), and plasminogen activator inhibitor type 1 (PAI-1). The hemodynamic environment was designed to mimic shear stress conditions at the distal anastomosis of a saphenous vein graft, a common site of intimal proliferation. Steady-state mRNA levels in experimental tubes were expressed relative to that in controls. No changes were observed in ET-1 mRNA after 1 and 24 hr, but a 50% decrease in experimental cultures was observed after 48 hr in the vascular simulating device. Similar results were ob- tained for ecNOS mRNA, although a subgroup (4 of 11) showed a significant decrease (>50%) by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annals of Vascular Surgery Springer Journals

The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile Flow and Cyclic Strain, In Vitro

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References (33)

Publisher
Springer Journals
Copyright
Copyright © 2000 by Annals of Vascular Surgery
Subject
Medicine & Public Health; Abdominal Surgery
ISSN
0890-5096
eISSN
1615-5947
DOI
10.1007/s100169910044
Publisher site
See Article on Publisher Site

Abstract

The Response of Adult Human Saphenous Vein Endothelial Cells to Combined Pressurized Pulsatile Flow and Cyclic Strain, In Vitro Olga I. Tsukurov, MD, Christopher J. Kwolek, MD, Gilbert J. L’Italien, PhD, Aziz Benbrahim, MD, Barbara B. Milinazzo, BS, Nancy E. Conroy, BS, Jonathan P. Gertler, MD, Roslyn W. Orkin, PhD, and William M. Abbott, MD, Boston, Massachusetts Adult human saphenous vein endothelial cells (HVEC) were cultured in a compliant tubular device and evaluated by Northern hybridization for the effects of combined pressurized pulsatile flow and cyclic strain on the expression of mRNAs for endothelin-1 (ET-1), endothelial cell nitric oxide synthase (ecNOS), tissue plasminogen activator (tPA), and plasminogen activator inhibitor type 1 (PAI-1). The hemodynamic environment was designed to mimic shear stress conditions at the distal anastomosis of a saphenous vein graft, a common site of intimal proliferation. Steady-state mRNA levels in experimental tubes were expressed relative to that in controls. No changes were observed in ET-1 mRNA after 1 and 24 hr, but a 50% decrease in experimental cultures was observed after 48 hr in the vascular simulating device. Similar results were ob- tained for ecNOS mRNA, although a subgroup (4 of 11) showed a significant decrease (>50%) by

Journal

Annals of Vascular Surgery – Springer Journals

Published: Feb 18, 2014

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