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C. Hildebrandt, Heiko Büth, H. Thielecke (2009)
Influence of cell culture media conditions on the osteogenic differentiation of cord blood-derived mesenchymal stem cells.Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 191 1
C. Rebelatto, A. Aguiar, M. Moretão, A. Senegaglia, P. Hansen, F. Barchiki, João Oliveira, J. Martins, Crisciele Kuligovski, Fernanda Mansur, A. Christofis, V. Amaral, P. Brofman, S. Goldenberg, L. Nakao, A. Correa (2008)
Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose TissueExperimental Biology and Medicine, 233
(2008)
Potapnev MP (2008) In vitro growth of human umbilical mesenchymal stem cells and their differentiation into chondrocytes and osteoblasts
K. Bieback, Susanne Kern, H. Klüter, H. Eichler (2004)
Critical Parameters for the Isolation of Mesenchymal Stem Cells from Umbilical Cord BloodSTEM CELLS, 22
A. Mohamed, A. Ibrahim, Manal El-Masry, I. Mansour, Mervat Khroshied, H. Gouda, R. Riad (2006)
Ex vivo expansion of stem cells: defining optimum conditions using various cytokines.Laboratory hematology : official publication of the International Society for Laboratory Hematology, 12 2
Kyung-Min Choi, Y. Seo, H. Yoon, K. Song, S. Kwon, H. Lee, J. Park (2008)
Effect of ascorbic acid on bone marrow-derived mesenchymal stem cell proliferation and differentiation.Journal of bioscience and bioengineering, 105 6
P. Kasten, I. Beyen, M. Egermann, Suda Aj, Moghaddam Aa, G. Zimmermann, R. Luginbühl (2008)
Instant stem cell therapy: characterization and concentration of human mesenchymal stem cells in vitro.European cells & materials, 16
Y. Tabata (2009)
Biomaterial technology for tissue engineering applicationsJournal of The Royal Society Interface, 6
D. Noël, D. Gazit, C. Bouquet, F. Apparailly, C. Bony, P. Plence, V. Millet, Gadi Turgeman, M. Perricaudet, J. Sany, C. Jorgensen (2004)
Short‐Term BMP‐2 Expression Is Sufficient for In Vivo Osteochondral Differentiation of Mesenchymal Stem CellsSTEM CELLS, 22
X Zhang, HA Awad, RJ O’Keefe, RE Guldberg, EM Schwarz (2008)
Engineering periosteum for structural bone graft healingClin Orthop Relat Res, 466
(2004)
Shortterm BMP-2 expression is sufficient for in vivo osteo-chondral
Ji-Soo Kim, Hyun-kyung Lee, Mi‐Ryung Kim, Pan-Kyeom Kim, Chan‐Wha Kim (2008)
Differentially Expressed Proteins of Mesenchymal Stem Cells Derived from Human Cord Blood (hUCB) during Osteogenic DifferentiationBioscience, Biotechnology, and Biochemistry, 72
P. Shetty, K. Bharucha, V. Tanavde (2007)
Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cellsCell Biology International, 31
T. Heino, T. Hentunen, H. Väänänen (2004)
Conditioned medium from osteocytes stimulates the proliferation of bone marrow mesenchymal stem cells and their differentiation into osteoblasts.Experimental cell research, 294 2
A. Lam, K. Li, X. Zhang, C. Li, T. Fok, Allan Chang, A. James, K. Tsang, P. Yuen (2001)
Preclinical ex vivo expansion of cord blood hematopoietic stem and progenitor cells: duration of culture; the media, serum supplements, and growth factors used; and engraftment in NOD/SCID miceTransfusion, 41
S. Wexler, C. Donaldson, P. Denning-Kendall, C. Rice, B. Bradley, J. Hows (2003)
Adult bone marrow is a rich source of human mesenchymal ‘stem’ cells but umbilical cord and mobilized adult blood are notBritish Journal of Haematology, 121
Ki-Soo Park, Yong‐Soon Lee, K. Kang (2006)
In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord bloodJournal of Veterinary Science, 7
(2004)
Ex vivo expansion of stem cells from umbilical cord : expression of cell adhesion molecule
I. Bojanić, B. Ćepulić (2006)
[Umbilical cord blood as a source of stem cells].Acta medica Croatica : casopis Hravatske akademije medicinskih znanosti, 60 3
E. Olmsted‐Davis, Z. Gugala, F. Camargo, F. Gannon, K. Jackson, Kirsten Kienstra, H. Shine, R. Lindsey, K. Hirschi, M. Goodell, M. Brenner, A. Davis (2003)
Primitive adult hematopoietic stem cells can function as osteoblast precursorsProceedings of the National Academy of Sciences of the United States of America, 100
G. Dravid, S. Rao (2002)
Ex Vivo Expansion of Stem Cells from Umbilical Cord Blood: Expression of Cell Adhesion MoleculesSTEM CELLS, 20
B. Kulterer, G. Friedl, A. Jandrositz, F. Sánchez-Cabo, A. Prokesch, Christine Paar, M. Scheideler, R. Windhager, K. Preisegger, Z. Trajanoski (2007)
Gene expression profiling of human mesenchymal stem cells derived from bone marrow during expansion and osteoblast differentiationBMC Genomics, 8
Chi-Hsien Liu, Mei-Ling Wu, Shiaw-Min Hwang (2007)
Optimization of serum free medium for cord blood mesenchymal stem cellsBiochemical Engineering Journal, 33
Xinping Zhang, H. Awad, R. O’Keefe, R. Guldberg, E. Schwarz (2008)
A Perspective: Engineering Periosteum for Structural Bone Graft HealingClinical Orthopaedics and Related Research, 466
X. Ju, Zhiyang Huang, Qing Shi, H. Hou, Chun-hong Duan (2005)
[Biological characteristics and induced differentiation ability of in vitro expanded umbilical cord blood mesenchymal stem cells].Zhonghua er ke za zhi = Chinese journal of pediatrics, 43 7
Trần Toại, Huynh Thao, Nguyen Thao, C. Gargiulo, P. Ngoc, P. Van, D. Strong (2010)
In vitro culture and differentiation of osteoblasts from human umbilical cord bloodCell and Tissue Banking, 11
F. Martn, Francisco Abada (2006)
from umbilical cord blood to rats with thioacetamide-induced liver cirrhosis
Lulu Lu, Yong‐jun Liu, Shao-guang Yang, Qin-jun Zhao, Xin Wang, W. Gong, Z. Han, Zhen-shu Xu, Yong-xin Lu, Delong Liu, Zhi-zhe Chen, Z. Han (2006)
Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis-supportive function and other potentials.Haematologica, 91 8
S. Kosmacheva, M. Volk, T. Yeustratenka, I. Severin, M. Potapnev (2008)
In vitro growth of human umbilical blood mesenchymal stem cells and their differentiation into chondrocytes and osteoblastsBulletin of Experimental Biology and Medicine, 145
Yong-sheng Zhou, Yunsong Liu, Jianshao Tan (2006)
Is 1, 25-dihydroxyvitamin D3 an ideal substitute for dexamethasone for inducing osteogenic differentiation of human adipose tissue-derived stromal cells in vitro?Chinese medical journal, 119 15
S. Deliloğlu-Gürhan, H. Vatansever, F. Ozdal-Kurt, I. Tuğlu (2006)
Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system.Acta histochemica, 108 1
K. Mareschi, E. Biasin, W. Piacibello, M. Aglietta, E. Madon, F. Fagioli (2001)
Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood.Haematologica, 86 10
Under specific culture conditions, umbilical cord blood derived mesenchymal stem cells (MSCs) can differentiate into osteogenic, adipogenic, and chondrogenic lineages. The purpose of the current study was to assess the differentiation potential of osteogenic umbilical cord blood derived hematopoietic stem cells (HSCs) and to develop an appropriate osteogenic differentiation medium for in vitro differentiation of umbilical cord blood derived HSCs. The study was conducted on 20 cord blood samples. The cells were cultured in osteogenic differentiating medium for 3 weeks. The HSCs differentiated into osteoblasts, which expressed osteoblast-associated genes (osteocalcin and bone sialoprotein), which were detected by RT-PCR. They showed alkaline phosphatase activity and a positive Alizarin red-S (AR-S) stain (calcium phosphate deposition). Umbilical cord blood is a rich source of hematopoietic stem cells that can be differentiated into osteoblasts; thus, it can be used for therapeutic strategies in the context of regenerative therapy.
Comparative Clinical Pathology – Springer Journals
Published: Oct 21, 2010
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