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The Mechanism of Antibacterial Action of the Lantibiotic Warnerin

The Mechanism of Antibacterial Action of the Lantibiotic Warnerin Evidence of membranotropic activity of the lantibiotic warnerin was obtained for warnerin-sensitive bacteria Staphylococcus cohnii VKM-3165. Warnerin attack led to increased bacterial respiratory activity and accumulation of reactive oxygen species resulting in the death of staphylococci. Warnerin caused dissipation of the transmembrane potential with release of potassium ions and ATP from the cells. Dissipation of the potential resulted in activation of bacterial autolysis, which depends on the value of the electrical component of the membrane potential. ΔΨ dissipation in the presence of valinomycin, a potassium transporter, almost neutralized the activation of the peptidoglycan hydrolase complex by warnerin. Electron microscopy of bacterial internal structure and scanning of the surface of warnerin-attacked cells of S. cohnii VKM-3165 confirmed the depth and irreversibility of warnerin action. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microbiology Springer Journals

The Mechanism of Antibacterial Action of the Lantibiotic Warnerin

Microbiology , Volume 91 (2) – Apr 1, 2022

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References (29)

Publisher
Springer Journals
Copyright
Copyright © Pleiades Publishing, Ltd. 2022. ISSN 0026-2617, Microbiology, 2022, Vol. 91, No. 2, pp. 184–191. © Pleiades Publishing, Ltd., 2022. Russian Text © The Author(s), 2022, published in Mikrobiologiya, 2022, Vol. 91, No. 2, pp. 217–225.
ISSN
0026-2617
eISSN
1608-3237
DOI
10.1134/s0026261722020059
Publisher site
See Article on Publisher Site

Abstract

Evidence of membranotropic activity of the lantibiotic warnerin was obtained for warnerin-sensitive bacteria Staphylococcus cohnii VKM-3165. Warnerin attack led to increased bacterial respiratory activity and accumulation of reactive oxygen species resulting in the death of staphylococci. Warnerin caused dissipation of the transmembrane potential with release of potassium ions and ATP from the cells. Dissipation of the potential resulted in activation of bacterial autolysis, which depends on the value of the electrical component of the membrane potential. ΔΨ dissipation in the presence of valinomycin, a potassium transporter, almost neutralized the activation of the peptidoglycan hydrolase complex by warnerin. Electron microscopy of bacterial internal structure and scanning of the surface of warnerin-attacked cells of S. cohnii VKM-3165 confirmed the depth and irreversibility of warnerin action.

Journal

MicrobiologySpringer Journals

Published: Apr 1, 2022

Keywords: antibacterial activity; autolysis activation; lantibiotics; membranotropic activity; mechanism of action

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