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The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants

The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants Strain MG1655+ hisG r hisL′-ΔΔ, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl transferase encoded by the mutant hisG r (E271K) was the main determinant of histidine production. A further increase in histidine production was achieved by the expression enhance of a mutant his operon containing hisG r through the deleting attenuator region (hisL′-Δ). An increase in the expression of the wildtype his operon did not result in histidine accumulation. Deletion of the transcriptional regulator gene purR increased the biomass produced and maintained the level of histidine production per cell under the fermentation conditions used. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

The directed modification of Escherichia coli MG1655 to obtain histidine-producing mutants

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683813020026
Publisher site
See Article on Publisher Site

Abstract

Strain MG1655+ hisG r hisL′-ΔΔ, purR, which produces histidine with a weight yield of approximately 12% from glucose, was constructed through directed chromosomal modifications of the laboratory Escherichia coli strain MG1655+, which has a known genome sequence. A feedback-resistant ATP-phosphoribosyl transferase encoded by the mutant hisG r (E271K) was the main determinant of histidine production. A further increase in histidine production was achieved by the expression enhance of a mutant his operon containing hisG r through the deleting attenuator region (hisL′-Δ). An increase in the expression of the wildtype his operon did not result in histidine accumulation. Deletion of the transcriptional regulator gene purR increased the biomass produced and maintained the level of histidine production per cell under the fermentation conditions used.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Feb 26, 2013

References

  • Metabolic Pathways
    Brenner, M.; Ames, B.N.
  • EcoSal—Escherichia coli and Salmonella: Cellular and Molecular Biology
    Winkler, M.E.; Ramos-Monta≠z, S.
  • Molecular Cloning: a Laboratory Manual
    Sambrook, J.; Russell, D.W.
  • Experiments in Molecular Genetics
    Miller, J.H.