Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells in cattle

The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells... This study used a combination of single antigen immunoperoxidase staining for bovine leukaemia virus (BLV) p24 capsid antigen and IgM to enumerate the peripheral blood mononuclear cells (PBMCs) infected with BLV and the number of B-cells, respectively. A significant relationship was found between the number of BLV-infected PBMCs and the number of circulating B-cells. A model was created that predicted the number of circulating B-cells using the number of BLV-infected PBMCs. These data also show that the percentage of B-cells in PBMCs preparations is not affected by 24 h of in vitro culture when LPS is added to the culture medium. Double antigen labelling showed that the majority of circulating B-cells were infected with BLV in some BLV-seropositive cattle that were not persistently lymphocytotic. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

The contribution of bovine leukaemia virus infected B-cells to the number of circulating B-cells in cattle

Loading next page...
 
/lp/springer-journals/the-contribution-of-bovine-leukaemia-virus-infected-b-cells-to-the-iVPvbkUMnX

References (59)

Publisher
Springer Journals
Copyright
Copyright © 1994 by Springer-Verlag London Limited
Subject
Medicine & Public Health; Pathology; Hematology; Oncology
eISSN
1433-2973
DOI
10.1007/BF00368275
Publisher site
See Article on Publisher Site

Abstract

This study used a combination of single antigen immunoperoxidase staining for bovine leukaemia virus (BLV) p24 capsid antigen and IgM to enumerate the peripheral blood mononuclear cells (PBMCs) infected with BLV and the number of B-cells, respectively. A significant relationship was found between the number of BLV-infected PBMCs and the number of circulating B-cells. A model was created that predicted the number of circulating B-cells using the number of BLV-infected PBMCs. These data also show that the percentage of B-cells in PBMCs preparations is not affected by 24 h of in vitro culture when LPS is added to the culture medium. Double antigen labelling showed that the majority of circulating B-cells were infected with BLV in some BLV-seropositive cattle that were not persistently lymphocytotic.

Journal

Comparative Clinical PathologySpringer Journals

Published: Sep 8, 2004

There are no references for this article.